Proteomic Characterization of the Venom of Five Bombus (Thoracobombus) Species

Venomous animals use venom, a complex biofluid composed of unique mixtures of proteins and peptides, to act on vital systems of the prey or predator. In bees, venom is solely used for defense against predators. However, the venom composition of bumble bees ( sp.) is largely unknown. The subgenus of...

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Bibliographic Details
Published in:Toxins 2017-11, Vol.9 (11), p.362
Main Authors: Barkan, Nezahat Pınar, Bayazit, Mustafa Bilal, Ozel Demiralp, Duygu
Format: Article
Language:English
Subjects:
2D-PAGE
Bees
Bombus
bumble bees
Ionization
Ions
MALDI-TOF MS
Mass spectrometry
Mass spectroscopy
Peptides
Predators
Prey
Proteins
proteomics
Species
Spots
Toxins
Venom
Venom toxins
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Summary:Venomous animals use venom, a complex biofluid composed of unique mixtures of proteins and peptides, to act on vital systems of the prey or predator. In bees, venom is solely used for defense against predators. However, the venom composition of bumble bees ( sp.) is largely unknown. The subgenus of sp. is a diverse subgenus represented by 14 members across Turkey. In this study, we sought out to proteomically characterize the venom of five species by using bottom-up proteomic techniques. We have obtained two-dimensional polyacrylamide gel (2D-PAGE) images of each species' venom sample. We have subsequently identified the protein spots by using matrix assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF MS). We have identified 47 proteins for , 32 for , 60 for , 39 for , and 35 for . Moreover, we illustrated that intensities of 2DE protein spots corresponding to putative venom toxins vary in a species-specific manner. Our analyses provide the primary proteomic characterization of five bumble bee species' venom composition.
ISSN:2072-6651
2072-6651
DOI:10.3390/toxins9110362