Chemopreventive effect of dietary inclusion with Crassocephalum rubens (Juss ex Jacq) leaf on N-methyl-N-nitrosourea (MNU)-induced colorectal carcinogenesis in Wistar rats

• Published in: Journal of functional foods 2019-12, Vol.63, p.103589, Article 103589
• Main Authors: Alhassan, Solomon Oguche, Atawodi, Sunday Ene-Ojo
• Format: Article
• Language: English
• Subjects: Antixidants; Carcinogenesis; Colorectal cancer; Crassocephalum rubens; Mismatch repair; Oxidative stress
• ISSN: 1756-4646; 2214-9414
• DOI: 10.1016/j.jff.2019.103589

[Display omitted] •C. rubens prevented oxidative stress indices and stabilized endogenous antioxidant status.•C. rubens reduced neutrophil-lymphocyte ratio and downregulated carcinoembryonic antigen.•C. rubens prevented MNU-induced colon necrosis and inflammation.•C. rubens sustained the expression of MLH1 protein, even under assault of chemical carcinogen. Chemoprevention leverages the ability of natural products to interfere with disease onset and progression. In this study, the preventive effect of dietary inclusion with Crassocephalum rubens at 0, 2.5, 5 and 10% levels on colorectal carcinogenesis initiated by N-methyl-N-nitrosourea (MNU) instillations in male Wistar rats was explored following 12 weeks of treatment. Results showed that dietary inclusion with C. rubens particularly at the 10% level significantly lowered lipid peroxidation, systemic inflammation, serum carcinoembryonic antigen (CEA), colon epithelial damage and aberrant expression of the mismatch repair protein MLH-1. The activity of superoxide dismutase and catalase were elevated in C. rubens fed subjects compared with MNU-only treatment controls. These observations correlated at p