Health Benefits of Lactobacillus gasseri CP2305 Tablets in Young Adults Exposed to Chronic Stress: A Randomized, Double-Blind, Placebo-Controlled Study

• Published in: Nutrients 2019-08, Vol.11 (8), p.1859
• Main Authors: Nishida, Kensei, Sawada, Daisuke, Kuwano, Yuki, Tanaka, Hiroki, Rokutan, Kazuhito
• Format: Article
• Language: English
• Subjects: Acids; Adult; Bacteria; Bifidobacterium - metabolism; Birth control; Chromogranin A - metabolism; Chronic Disease; Clinical trials; Communications systems; Double-Blind Method; Double-blind studies; fecal microbiota; Female; Gastrointestinal Microbiome; Healthy Volunteers; healthy young adults; heat-inactivated Lactobacillus gasseri CP2305; Homeostasis; Humans; Hypothalamic-pituitary-adrenal axis; Intestine; Irritable bowel syndrome; Lactobacillus gasseri; Latency; Male; Mental disorders; Mental health; Microbiota; Probiotics; Probiotics - administration & dosage; psychobiotics; RNA, Ribosomal, 16S - analysis; Saliva - chemistry; Sensory neurons; Sleep; Sleep and wakefulness; sleep quality; Sleep Wake Disorders - microbiology; Sleep Wake Disorders - psychology; Sleep Wake Disorders - therapy; Streptococcus - metabolism; Stress; Stress, Psychological - microbiology; Stress, Psychological - psychology; Stress, Psychological - therapy; Students, Medical - psychology; Tablets; Treatment Outcome; Vagus nerve; Young Adult; Young adults
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu11081859

Short-term administration of CP2305 improves stress-associated symptoms and clinical symptoms in healthy young adults and in patients with irritable bowel syndrome, respectively. We evaluated the efficacy and health benefits of the long-term use of a tablet containing heat-inactivated, washed CP2305 (CP2305) in healthy young adults. Sixty Japanese medical students (41 men and 19 women) preparing for the national examination for medical practitioners ingested CP2305-containing or placebo tablets once daily for 24 weeks. Intake of the CP2305 tablet significantly reduced anxiety and sleep disturbance relative to placebo, as quantitated by the Spielberger State-Trait Anxiety Inventory and the Pittsburgh Sleep Quality Index. Single-channel sleep electroencephalograms show that CP2305 significantly shortened sleep latency and wake time after sleep onset and increased the delta power ratio in the first sleep cycle. CP2305 also significantly lowered salivary chromogranin A levels compared with placebo. Furthermore, 16S rRNA gene sequencing of participant feces demonstrated that CP2305 administration attenuated the stress-induced decline of spp. and the stress-induced elevation of spp. We conclude that the long-term use of CP2305-containing tablets may improve the mental state, sleep quality, and gut microbiota of healthy adults under stressful conditions.