Vitamin D protects podocytes from autoantibodies induced injury in lupus nephritis by reducing aberrant autophagy

The aim of this study was to investigate whether vitamin D plays a protective role in podocyte injury induced by autoantibodies purified from the serum of patients with lupus nephritis (LN) via reducing aberrant autophagy. Autophagic activities of renal tissues of patients with LN were evaluated und...

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Bibliographic Details
Published in:Arthritis research & therapy 2019-01, Vol.21 (1), p.19-19, Article 19
Main Authors: Yu, Qi, Qiao, Yingjin, Liu, Dongwei, Liu, Fengxun, Gao, Congcong, Duan, Jiayu, Liang, Lulu, Di, Xueqi, Yuan, Yi, Gao, Yukui, Cui, Siwan, Qin, Yilu, Li, Tianfang, Zheng, Zhaohui, Liu, Zhangsuo
Format: Article
Language:English
Subjects:
Adenoviruses
Adolescent
Adult
Antibodies
Arthritis
Autoantibodies
Autoantibodies - blood
Autoantibodies - drug effects
Autoimmune diseases
Autoimmunity
Autophagy
Autophagy (Cytology)
Autophagy - drug effects
Autophagy - physiology
Care and treatment
Cell Line, Transformed
Child
Disease
Female
Hepatitis
Homeostasis
Humans
Immunoglobulin G
Immunoglobulins
Kidney diseases
Kidneys
Kinases
Lupus
Lupus nephritis
Lupus Nephritis - blood
Lupus Nephritis - drug therapy
Lupus Nephritis - pathology
Male
Middle Aged
Nephritis
Nephrology
Novels
Pathogenesis
Podocyte
Podocytes - drug effects
Podocytes - metabolism
Podocytes - ultrastructure
Polymerase chain reaction
Vitamin D
Vitamin D - pharmacology
Vitamin deficiency
Vitamins
Young Adult
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Summary:The aim of this study was to investigate whether vitamin D plays a protective role in podocyte injury induced by autoantibodies purified from the serum of patients with lupus nephritis (LN) via reducing aberrant autophagy. Autophagic activities of renal tissues of patients with LN were evaluated under transmission electronic microscope (TEM). Immunoglobulin G (IgG) from patients with LN was purified to induce human podocyte injury, and the role of vitamin D in injury was observed. Podocytes were observed under TEM, autophagic activity was evaluated by western blot analysis and quantitative real-time polymerase chain reaction, and mRFP-GFP-LC3B adenovirus was infected into human podocytes in vitro. Significantly higher autophagic levels were observed in patients with LN (P
ISSN:1478-6362
1478-6354
1478-6362
DOI:10.1186/s13075-018-1803-9