Amniotic-Fluid Stem Cells : Growth Dynamics and Differentiation Potential after a CD-117-Based Selection Procedure

Amniotic fluid (AF) has become an interesting source of fetal stem cells. However, AF contains heterogeneous and multiple, partially differentiated cell types. After isolation from the amniotic fluid, cells were characterized regarding their morphology and growth dynamics. They were sorted by magnet...

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Bibliographic Details
Published in:Stem cells international 2011-01, Vol.2011 (2011), p.1-12
Main Authors: Arnhold, S., Glüer, S., Hartmann, K., Raabe, O., Addicks, Klaus, Wenisch, S., Hoopmann, M.
Format: Article
Language:English
Subjects:
Amniotic fluid
Growth
Health aspects
Neurons
Physiological aspects
Stem cells
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Summary:Amniotic fluid (AF) has become an interesting source of fetal stem cells. However, AF contains heterogeneous and multiple, partially differentiated cell types. After isolation from the amniotic fluid, cells were characterized regarding their morphology and growth dynamics. They were sorted by magnetic associated cell sorting using the surface marker CD 117. In order to show stem cell characteristics such as pluripotency and to evaluate a possible therapeutic application of these cells, AF fluid-derived stem cells were differentiated along the adipogenic, osteogenic, and chondrogenic as well as the neuronal lineage under hypoxic conditions. Our findings reveal that magnetic associated cell sorting (MACS) does not markedly influence growth characteristics as demonstrated by the generation doubling time. There was, however, an effect regarding an altered adipogenic, osteogenic, and chondrogenic differentiation capacity in the selected cell fraction. In contrast, in the unselected cell population neuronal differentiation is enhanced.
ISSN:1687-966X
1687-9678
1687-9678
DOI:10.4061/2011/715341