Anticandidal and In vitro Anti-Proliferative Activity of Sonochemically synthesized Indium Tin Oxide Nanoparticles

• Published in: Scientific reports 2020-02, Vol.10 (1), p.3228-9, Article 3228
• Main Authors: Rehman, Suriya, Asiri, Sarah Mousa, Khan, Firdos Alam, Jermy, B. Rabindran, Ravinayagam, Vijaya, Alsalem, Zainab, Jindan, Reem Al, Qurashi, Ahsanulhaq
• Format: Article
• Language: English
• Subjects: 13/106; 631/61/350/354; 639/925/350/354; Antifungal Agents - pharmacology; Biofilms - drug effects; Candida; Candida - drug effects; Candida - ultrastructure; Cell Proliferation - drug effects; Cell Shape - drug effects; Cell viability; Colorectal carcinoma; Crystallization; Dosage; HCT116 Cells; HEK293 Cells; Humanities and Social Sciences; Humans; Hyphae - drug effects; Hyphae - growth & development; Indium; Microbial Sensitivity Tests; Minimum inhibitory concentration; multidisciplinary; Nanoparticles; Nanoparticles - chemistry; Nanoparticles - ultrastructure; Scanning electron microscopy; Science; Science (multidisciplinary); Sonication; Tin; Tin - chemistry; Tin Compounds - chemical synthesis; Tin oxide; Transmission electron microscopy; X-Ray Diffraction
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-60295-w

The present work demonstrates the synthesis, characterization and biological activities of different concentrations of tin doped indium oxide nanoparticles (Sn doped In 2 O 3 NPs), i.e., (Sn/In = 5%, 10% and 15%). We have synthesized different size (38.11 nm, 18.46 nm and 10.21 nm) of Sn doped In 2 O 3 NPs. by using an ultra-sonication process. The Sn doped In 2 O 3 NPs were characterized by by x-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM) which confirmed the successful doping of tin (Sn) with Indium oxide (In 2 O 3 ). Anticandidal activity was performed by standard agar dilution method using Candida albicans for the study. The minimum inhibitory/fungicidal concentration (MIC/MFC) values recorded were, 8 & >8 mg/ml for pure In 2 O 3 NPs, 4 & 8 mg/ml for 5%, 2 & 8 mg/ml for 10%, whereas 1 & >4 mg/ml for 15% Sn doped In 2 O 3 NPs, respectively. The topographical alteration caused by Sn doped In 2 O 3 NPs on Candida cells, was clearly observed by SEM examination. A significant enhancement in anticandidal activity was seen, when Candida cells were exposed to (Sn/In = 5%, 10% and 15%). Moreover, we have also evaluated the impact of Sn-In 2 O 3 NPs on human colorectal carcinoma cells (HCT-116). The results demonstrated that Sn-In 2 O 3 NPs (Sn/In = 5%, 10% and 15%), caused dose dependent decrease in the cancer cell viability as the low dosage (2.0 mg/mL) showed 62.11% cell viability, while 4.0, 8.0, 16.0, 32.0 mg/mL dosages showed 20.45%, 18.25%, 16.58%, and 15.58% cell viability. In addition, the treatment of Sn-In 2 O 3 NPs also showed significant cellular and anatomical changes in cancer cells as examined by microscopes. We have also examined the impact of Sn-In 2 O 3 NPs (5%, 10%, 15%) on normal cells (HEK-293) and the results demonstrate that Sn-In 2 O 3 NPs did not reduce the cell viability of normal cells.