Screening and Antifungal Activity of a β-Carboline Derivative against Cryptococcus neoformans and C. gattii

• Published in: International journal of microbiology 2019, Vol.2019 (2019), p.1-8
• Main Authors: Souza, João Vicente Braga de, Montoia, Andreia, Souza, Érica Simplício, Silva, Marcia de Jesus Amazonas da, Lima, Emerson Silva, Cruz, Kátia Santana, Pohlit, Adrian Martin
• Format: Article
• Language: English
• Subjects: Alkaloids; Antibiotics; Antifungal activity; Antimicrobial agents; Candida albicans; Carbazole; Carbazoles; Cell walls; Cryptococcosis; Cryptococcus; Cryptococcus neoformans; Cytotoxicity; Derivatives; Ergosterol; Fibroblasts; Fungal diseases; Fungal infections; Fungi; Fungicides; Genotypes; Infections; Lead compounds; Leakage; Meningitis; Microbiology; Minimum inhibitory concentration; Nucleic acids; Pathogenicity; Pathogens; Plant diseases; Screening; Toxicity; Toxicology
• ISSN: 1687-918X; 1687-9198
• DOI: 10.1155/2019/7157845

Background. Cryptococcosis is a fungal disease of bad prognosis due to its pathogenicity and the toxicity of the drugs used for its treatment. The aim of this study was to investigate the medicinal potential of carbazole and β-carboline alkaloids and derivatives against Cryptococcus neoformans and C. gattii. Methods. MICs were established in accordance with the recommendations of the Clinical and Laboratory Standards Institute for alkaloids and derivatives against C. neoformans and C. gattii genotypes VNI and VGI, respectively. A single active compound was further evaluated against C. neoformans genotypes VNII, VNIII, and VNIV, C. gattii genotypes VGI, VGIII, and VGIV, Candida albicans ATCC 36232, for cytotoxicity against the MRC-5 lineage of human fibroblasts and for effects on fungal cells (cell wall, ergosterol, and leakage of nucleic acids). Results. Screening of 11 compounds revealed 8-nitroharmane as a significant inhibitor (MIC 40 μg/mL) of several C. neoformans and C. gattii genotypes. It was not toxic to fibroblasts (IC50 > 50 µg/mL) nor did it alter fungal cell walls or the concentration of ergosterol in C. albicans or C. neoformans. It increased leakage of substances that absorb at 260 nm. Conclusions. The synthetic β-carboline 8-nitroharmane significantly inhibits pathogenic Cryptococcus species and is interesting as a lead compound towards new therapy for Cryptococcus infections.