Overexpression of Cassava MeAnn2 Enhances the Salt and IAA Tolerance of Transgenic Arabidopsis

Annexins are a superfamily of soluble calcium-dependent phospholipid-binding proteins that have considerable regulatory effects in plants, especially in response to adversity and stress. The Arabidopsis thaliana AtAnn1 gene has been reported to play a significant role in various abiotic stress respo...

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Bibliographic Details
Published in:Plants (Basel) 2021-05, Vol.10 (5), p.941
Main Authors: Lin, Xuejun, Li, Ruimei, Zhou, Yangjiao, Tang, Fenlian, Wang, Yajie, Lu, Xiaohua, Wang, Shijia, Yao, Yuan, Liu, Jiao, Hu, Xinwen, Guo, Jianchun
Format: Article
Language:English
Subjects:
Abiotic stress
Acetic acid
Amino acids
annexin
Annexins
Arabidopsis
Arabidopsis thaliana
Binding
Binding sites
Calcium
Calcium ions
Cassava
Cell membranes
Cellular stress response
Cloning
Cotton
Cytoplasm
Domains
Genes
genetic transformation
Genomes
Indoleacetic acid
Localization
Malondialdehyde
Manihot esculenta
MeAnn2
Metabolism
Phospholipids
Plant growth
Proteins
Salinity tolerance
Salt tolerance
Signal transduction
Signaling
Sodium chloride
stress analysis
Tobacco
Transgenic plants
Tryptophan
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Summary:Annexins are a superfamily of soluble calcium-dependent phospholipid-binding proteins that have considerable regulatory effects in plants, especially in response to adversity and stress. The Arabidopsis thaliana AtAnn1 gene has been reported to play a significant role in various abiotic stress responses. In our study, the cDNA of an annexin gene highly similar to AtAnn1 was isolated from the cassava genome and named MeAnn2. It contains domains specific to annexins, including four annexin repeat sequences (I–IV), a Ca2+-binding sequence, Ca2+-independent membrane-binding-related tryptophan residues, and a salt bridge-related domain. MeAnn2 is localized in the cell membrane and cytoplasm, and it was found to be preferentially expressed in the storage roots of cassava. The overexpression of MeAnn2 reduced the sensitivity of transgenic Arabidopsis to various Ca2+, NaCl, and indole-3-acetic acid (IAA) concentrations. The expression of the stress resistance-related gene AtRD29B and auxin signaling pathway-related genes AtIAA4 and AtLBD18 in transgenic Arabidopsis was significantly increased under salt stress, while the Malondialdehyde (MDA) content was significantly lower than that of the control. These results indicate that the MeAnn2 gene may increase the salt tolerance of transgenic Arabidopsis via the IAA signaling pathway.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants10050941