Efficacy of EDTA and HEDP Chelators in the Removal of Mature Biofilm of Enterococcus faecalis by PUI and XPF File Activation

Biofilm removal from the root canal during endodontic treatment is necessary to prevent further complications. Irrigation is essential to success. Several irrigants have been proposed without a proper comparison. The aim of the study is to compare the antibacterial capacity of different activated ir...

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Bibliographic Details
Published in:Dentistry journal 2021-04, Vol.9 (4), p.41
Main Authors: Álvarez-Sagües, Alejandro, Herce, Nerea, Amador, Ulises, Llinares-Pinel, Francisco, Nistal-Villan, Estanislao, Presa, Jesús, Álvarez, Laura, Azabal, Magdalena
Format: Article
Language:English
Subjects:
Acetic acid
Antiinfectives and antibacterials
Antimicrobial agents
Bacteria
Biofilms
Chelating agents
Complications
Dental roots
Dentistry
Dilution
Edetic acid
EDTA
Endo-XP finisher (XPF)
Endodontics
Enterococcus faecalis
ethydronic acid (HEDP)
Ethylenediaminetetraacetic acids
Microorganisms
passive ultrasonic activation (PUI)
Root canals
Sodium hypochlorite
Subgroups
Teeth
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Summary:Biofilm removal from the root canal during endodontic treatment is necessary to prevent further complications. Irrigation is essential to success. Several irrigants have been proposed without a proper comparison. The aim of the study is to compare the antibacterial capacity of different activated irrigants using passive ultrasonic activation (PUI) or XP-Endo finisher (XPF). A total of 100 instrumented teeth were incubated in an Eppendorf tube containing 0.5 McFarland of and incubated for 2 weeks at 37 °C. Roots were divided into 5 groups ( = 20) according to the irrigant type: ethylenediaminetetraacetic acid (EDTA) (17%), ethydronic acid (HEDP) (9%) mixed with 5.25% sodium hypochlorite (NaOCl), EDTA (17%) mixed with 5.25% NaOCl, PBS, and a control group. Each group was divided into two subgroups ( = 10): PUI and XPF. As compared to the untreated control group, the irrigators included in the study had a significant effect in bacteria reduction. The obtained results show HEDP to be the most effective irrigant, since no bacteria were recovered after treatment of this group, followed by EDTA mixed with NaOCl and, finally, the EDTA-irrigated group. HEDP is the best irrigating agent in combination with XPF or PUI file activation to eliminate bacteria in our experimental model.
ISSN:2304-6767
2304-6767
DOI:10.3390/dj9040041