Effect of antioxidant treatment with n-acetylcysteine and swimming on lipid expression of sebaceous glands in diabetic mice

• Published in: Scientific reports 2021-06, Vol.11 (1), p.11924-11924, Article 11924
• Main Authors: Santos, Geovane Ribeiro, Cunha, Marcelo Rodrigues, Caldeira, Eduardo José, Galdeano, Ewerton Alexandre, Prudente, Raphael Cruz Seabra, Pinto, Clóvis Antonio Lopes
• Format: Article
• Language: English
• Subjects: 631/1647; 631/443; 631/45; 631/80; 631/92; 692/53; 692/699; Acetylcysteine; Acetylcysteine - pharmacology; Animals; Antioxidants; Antioxidants - pharmacology; Blood Glucose - metabolism; Deoxyribonucleic acid; Diabetes; Diabetes mellitus; Diabetes Mellitus, Type 1 - blood; Diabetes Mellitus, Type 1 - metabolism; DNA; Female; Histochemistry; Histology; Humanities and Social Sciences; Humans; Immunohistochemistry; Inflammation; Lipids; Lipids - biosynthesis; Mice; Mice, Inbred BALB C; Mice, Inbred NOD; Morphology; multidisciplinary; Perilipin-2 - metabolism; Physical training; Science; Science (multidisciplinary); Sebaceous gland; Sebaceous Glands - anatomy & histology; Sebaceous Glands - drug effects; Sebaceous Glands - metabolism; Skin - drug effects; Skin - metabolism; Statistical analysis; Swimming; Swimming - physiology; Tumors
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-021-91459-x

The sebaceous gland (SG) is involved in different inflammatory, infectious and neoplastic processes of the skin and can be related to specific diseases, e.g., diabetes mellitus. Sometimes, the histological diagnosis requires complementary tests due to the ability of diseases to mimic other tumors. We evaluated the sebaceous gland density in Non-obese diabetic mice to analyze the N-acetylcystein effects and swimming exercise treatment in sebaceous glands healing, using specific staining in histochemistry and immunohistochemistry reactions in the identification of the lipid expression in the sebaceous gland. We investigated the intracytoplasmic lipid expression and analysis of gland density from SG in dorsal skin samples from the Non-obese diabetic (NOD mice) and diabetic animals submitted to antioxidant treatment and physical exercise. For histological analysis of the sebaceous glands, specific staining in histochemistry with sudan black and immunohistochemistry reaction with adipophilin were used in the evaluation. Statistical analysis showed significant proximity between the values of the control group and the diabetic group submitted to the swimming exercise (DS group) and similar values between the untreated diabetic group (UD group) and diabetic group treated with the antioxidant N-acetylcysteine (DNa group), which did not prevent possible differences where p