Molecular Assessment of Glutathion S-Transferases (GSTT1 & GSTM1) Genotypes in HCV Infection

Glutathione S-Transferases Mu-1 (GSTM1) and Glutathione S-Transferases Theta-1 (GSTT1) are expressed frequently in liver cell and have been observed to have prominent role in neutralizing reactive oxygen species producing as results of normal metabolism, stress or infection. Oxidative stress plays a...

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Bibliographic Details
Published in:Pure and applied biology 2013-09, Vol.2 (3), p.89-95
Main Author: ullah, Farman
Format: Article
Language:English
Subjects:
Chloroform
Detoxification
Genetic engineering
Genotypes
GST
HCV
Hepatitis
Hepatitis C virus
Infections
Mutation
Null genotype
Oxidative stress
Phenols
Socioeconomic factors
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Summary:Glutathione S-Transferases Mu-1 (GSTM1) and Glutathione S-Transferases Theta-1 (GSTT1) are expressed frequently in liver cell and have been observed to have prominent role in neutralizing reactive oxygen species producing as results of normal metabolism, stress or infection. Oxidative stress plays an important role in the pathogenesis of HCV-induced damage because Glutathione S-Transferases directly involved in the detoxification of reactive oxygen species. A total of 76 blood samples were collected. Human genomic DNA was extracted through phenol/chloroform method and GSTs genotype was analyzed observed. Among 56 patient's samples, GSTT1(-), GSTM1(-) and null genotype were 21.05%, 28.95%, 23.69% respectively while 26.3% of individuals showed normal GST pattern. The individuals younger than 31 years showed GSTT1(-) 2.63%, GSTM1(-) 6.58% and null genotype 3.95 %. It has been concluded that increase in age, increases the chances of both GSTT1 and GSTM1 deletion(-), irrespective of null genotypes, however chronic HCV infection produce frequent GST's null genotypes.
ISSN:2304-2478
2304-2478
DOI:10.19045/bspab.2013.23003