Identification of a Novel Biosurfactant with Antimicrobial Activity Produced by Rhodococcus opacus R7

members excrete secondary metabolites, especially compounds which act as biosurfactants. In this work, we demonstrated the ability of R7 to produce a novel bioactive compound belonging to the class of biosurfactants with antimicrobial properties during the growth on naphthalene. Chemical and biochem...

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Bibliographic Details
Published in:Microorganisms (Basel) 2022-02, Vol.10 (2), p.475
Main Authors: Zampolli, Jessica, De Giani, Alessandra, Di Canito, Alessandra, Sello, Guido, Di Gennaro, Patrizia
Format: Article
Language:English
Subjects:
Amino acid composition
Amino acids
Antibiotics
Antiinfectives and antibacterials
Antimicrobial activity
Antimicrobial agents
antimicrobials
Bacteria
Bioactive compounds
biodegradation
Biosurfactants
biosynthesis
Carbohydrates
Carbon
Dilution
E coli
Hydrophobicity
Iodine
Mass spectrometry
Mass spectroscopy
Metabolites
Micelles
Microorganisms
Minimum inhibitory concentration
Molecular weight
Naphthalene
NMR
Nuclear magnetic resonance
Oil recovery
Peptides
Rhodococcus
Rhodococcus opacus
Secondary metabolites
Solvents
Surface tension
Surfactants
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Summary:members excrete secondary metabolites, especially compounds which act as biosurfactants. In this work, we demonstrated the ability of R7 to produce a novel bioactive compound belonging to the class of biosurfactants with antimicrobial properties during the growth on naphthalene. Chemical and biochemical analyses of the isolated compound demonstrated that the biosurfactant could be classified as a hydrophobic peptide. The ESI-full mass spectrometry revealed that the isolated biosurfactant showed a molecular weight of 1292 Da and NMR spectra evidenced the composition of the following amino acid residues: Ala, Thr, Asp, Gly, Ser. Surfactant activity of the . R7 compound was quantified by the critical micelle dilution (CMD) method and the critical micelle concentration (CMC) was estimated around 20 mg L with a corresponding surface tension of 48 mN m . Moreover, biological assays demonstrated that . R7 biosurfactant peptide exhibited antimicrobial activity against ATCC 29522 and ATCC 6538 with the minimum inhibition growth concentration (MIC) values of 2.6 mg mL and 1.7 mg mL , respectively. In this study for the first time, a hydrophobic peptide with both biosurfactant and antimicrobial activity was isolated from a bacterium belonging to genus.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms10020475