Research on the Region-Growing and Segmentation Technology of Micro-Particle Microscopic Images Based on Color Features

• Published in: Symmetry (Basel) 2021-12, Vol.13 (12), p.2325
• Main Authors: Hu, Xinyu, Chen, Qi, Ye, Xuhui, Zhang, Daode, Tang, Yuxuan, Ye, Jun
• Format: Article
• Language: English
• Subjects: Accuracy; Algorithms; Blood; Butterflies & moths; Color; color information; detection of silkworm disease; fuzzy enhancement; Gray scale; HSV color space; Image contrast; Image enhancement; Image segmentation; Impurities; Machine vision; Methods; micro-particles; Microparticles; Microscopy; region growing; Silkworms; Spores; Watersheds
• ISSN: 2073-8994; 2073-8994
• DOI: 10.3390/sym13122325

Silkworm microparticle disease is a legal quarantine standard in the detection of silkworm disease all over the world. The current common detection method, the Pasteur manual microscopy method, has a low detection efficiency all over the world. The low efficiency of the current Pasteur manual microscopy detection method makes the application of machine vision technology to detect microparticle spores an important technology to advance silkworm disease research. For the problems of the low contrast, different illumination conditions and complex image background of microscopic images of the ellipsoidal symmetrical shape of silkworm microparticle spores collected in the detection solution, a region growth segmentation method based on microparticle color and grayscale information is proposed. In this method, the fuzzy contrast enhancement algorithm is used to enhance the color information of micro-particles and improve the discrimination between the micro-particles and background. In the HSV color space with stable color, the color information of micro-particles is extracted as seed points to eliminate the influence of light and reduce the interference of impurities to locate the distribution area of micro-particles accurately. Combined with the neighborhood gamma transformation, the highlight feature of the micro-particle target in the grayscale image is enhanced for region growing. Mea6nwhile, the accurate and complete micro-particle target is segmented from the complex background, which reduces the background impurity segmentation caused by a single feature in the complex background. In order to evaluate the segmentation performance, we calculate the IOU of the microparticle sample image segmented by this method with its corresponding true value image, and the experiments show that the combination of color and grayscale features using the region growth technique can accurately and completely segment the microparticle target in complex backgrounds with a segmentation accuracy IOU as high as 83.1%.