Multiple Isolates from Aids Patients: Aspects of an Analysis by a Genotypic Marker and Antimicrobial Susceptibilities Variations

• Published in: Memórias do Instituto Oswaldo Cruz 2000-10, Vol.95 (5), p.729-732
• Main Authors: Saad, M H, Telles, M A, Porfirio, F, Ferrazoli, L, de Souza Fonseca, L, Johnson, Jr, W, Riley, L W
• Format: Article
• Language: English
• Subjects: AIDS-Related Opportunistic Infections - microbiology; AIDS/HIV; DNA Fingerprinting; Genetic Markers; Genotype; Human immunodeficiency virus 1; Humans; Microbial Sensitivity Tests; Mycobacterium avium; Mycobacterium avium Complex - drug effects; Mycobacterium avium Complex - genetics; Mycobacterium avium, polyclonal infection, susceptibility test; Mycobacterium avium-intracellulare Infection - microbiology; PARASITOLOGY; Phenotype; polyclonal infection; Polymorphism, Restriction Fragment Length; susceptibility test; TROPICAL MEDICINE
• ISSN: 1678-8060; 0074-0276; 0074-0276; 1678-8060
• DOI: 10.1590/S0074-02762000000500021

Twenty-one Mycobacterium avium multisolates, from ten human immunodeficiency virus-infected patients, were typed by restriction fragment length polymorphism using as marker the IS1245 and characterized by minimum inhibitory concentration for nine different antibiotics. Two out of four patients harboring multisolates with different fingerprint profile, were therefore considered as having a polyclonal infection, since their isolates were taken from sterile site. This result confirms that polyclonal infection caused by M. avium occurs with a nonnegligenciable frequency. Analyzing the multisolates susceptibility profile of each patient it was observed that most of them were infected with strains having appreciably different antimicrobial susceptibility patterns, no matter what the genotypic pattern of the strains was. These results have strong implication for the treatment of the patients.