Whole Genome Analysis of Sugarcane Root-Associated Endophyte Pseudomonas aeruginosa B18-A Plant Growth-Promoting Bacterium With Antagonistic Potential Against Sporisorium scitamineum

Sugarcane smut is a significant fungal disease that causes a major loss in sugar yield and quality. In this study, we isolated an endophytic strain B18 from a sugarcane root, which showed plant growth-promotion, hydrolytic enzyme production, antifungal activity against sugarcane pathogens ( ), and t...

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Published in:Frontiers in microbiology 2021-02, Vol.12, p.628376-628376
Main Authors: Singh, Pratiksha, Singh, Rajesh Kumar, Guo, Dao-Jun, Sharma, Anjney, Singh, Ram Nageena, Li, Dong-Ping, Malviya, Mukesh K, Song, Xiu-Peng, Lakshmanan, Prakash, Yang, Li-Tao, Li, Yang-Rui
Format: Article
Language:English
Subjects:
biocontrol
biotic stress
endophyte
genome sequence
Microbiology
plant growth promotion
Pseudomonas aeruginosa
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Summary:Sugarcane smut is a significant fungal disease that causes a major loss in sugar yield and quality. In this study, we isolated an endophytic strain B18 from a sugarcane root, which showed plant growth-promotion, hydrolytic enzyme production, antifungal activity against sugarcane pathogens ( ), and the presence of , , and antibiotic genes ( , and ) under conditions. BIOLOG phenotypic profiling of B18 established its ability to use various carbon and nitrogen sources and tolerate a range of pH and osmotic and temperature stresses. Whole-genome analysis of B18, identified as , showed that it consists of a single circular chromosome of 6,490,014 bp with 66.33% GC content. Genome annotation has identified 5,919 protein-coding genes, and 65 tRNA, and 12 rRNA genes. The B18 genome encodes genes related to ethylene, nitrogen ( , , , and ), and phosphate ( and ) metabolism and produce indole-3-acetic acid and siderophores. This also includes genes encoding hydrolases and oxidoreductases, those associated with biocontrol mechanisms ( , , , and ), colonization ( and ), and biofilm formation ( , , , and ), and those associated with metabolism of secondary metabolites. Collectively, these results suggest a role for B18 in plant growth enhancement and biocontrol mechanisms. The B18 strain was found to be an efficient colonizer in sugarcane; it can improve growth through modulation of plant hormone production and enhanced host-plant resistance to smut pathogen in a smut-susceptible sugarcane variety (Yacheng71-374). These biocontrol and plant growth promotion properties of B18 area are discussed in this report.
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2021.628376