Quantitative Proteomic Analysis of MCM3 in ThinPrep Samples of Patients with Cervical Preinvasive Cancer

Triage methods for cervical cancer detection show moderate accuracy and present considerable false-negative and false-positive result rates. A complementary diagnostic parameter could help improve the accuracy of identifying patients who need treatment. A pilot study was performed using a targeted p...

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Bibliographic Details
Published in:International journal of molecular sciences 2023-06, Vol.24 (13), p.10473
Main Authors: Köse, Büşra, Laar, Ralf van de, Beekhuizen, Heleen van, Kemenade, Folkert van, Baykal, Ahmet Tarik, Luider, Theo, Güzel, Coşkun
Format: Article
Language:English
Subjects:
Analysis
Biomarkers
Biopsy
Cancer
Cancer patients
Cancer screening
Care and treatment
Cellular biology
Cervical cancer
Cervix
Clinical medicine
Cytology
Diagnosis
Early Detection of Cancer
early diagnosis
Female
Histology
Human papillomavirus
Humans
Infections
Malignancy
Medical screening
Minichromosome Maintenance Complex Component 3
minichromosome maintenance-3 (MCM3)
Papillomaviridae - genetics
Papillomavirus Infections - pathology
Parallel Reaction Monitoring (PRM)
Patients
Pilot Projects
protein biomarker
Proteins
Proteomics
Scientific equipment and supplies industry
Squamous cells
ThinPrep
Uterine Cervical Dysplasia - pathology
Uterine Cervical Neoplasms - pathology
Womens health
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Summary:Triage methods for cervical cancer detection show moderate accuracy and present considerable false-negative and false-positive result rates. A complementary diagnostic parameter could help improve the accuracy of identifying patients who need treatment. A pilot study was performed using a targeted proteomics approach with opportunistic ThinPrep samples obtained from women collected at the hospital's outpatient clinic to determine the concentration levels of minichromosome maintenance-3 (MCM3) and envoplakin (EVPL) proteins. Forty samples with 'negative for intraepithelial lesion or malignancy' (NILM), 21 samples with 'atypical squamous cells of undetermined significance' (ASC-US), and 33 samples with 'low-grade squamous intraepithelial lesion and worse' (≥LSIL) were analyzed, using cytology and the patients' histology reports. Highly accurate concordance was obtained for gold-standard-confirmed samples, demonstrating that the MCM3/EVPL ratio can discriminate between non-dysplastic and dysplastic samples. On that account, we propose that MCM3 and EVPL are promising candidate protein biomarkers for population-based cervical cancer screening.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms241310473