GhWRKY70D13 Regulates Resistance to Verticillium dahliae in Cotton Through the Ethylene and Jasmonic Acid Signaling Pathways

wilt caused by is a destructive cotton disease causing severe yield and quality losses worldwide. WRKY transcription factors play important roles in plant defense against pathogen infection. However, little has been reported on the functions of WRKYs in cotton's resistance to . Here, we identif...

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Bibliographic Details
Published in:Frontiers in plant science 2020-02, Vol.11, p.69-69
Main Authors: Xiong, Xiang-Peng, Sun, Shi-Chao, Zhang, Xin-Yu, Li, Yan-Jun, Liu, Feng, Zhu, Qian-Hao, Xue, Fei, Sun, Jie
Format: Article
Language:English
Subjects:
ethylene
GhWRKY70D13
Gossypium hirsutum
jasmonic acid
Plant Science
ribonucleic acid interference
Verticillium dahliae
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Summary:wilt caused by is a destructive cotton disease causing severe yield and quality losses worldwide. WRKY transcription factors play important roles in plant defense against pathogen infection. However, little has been reported on the functions of WRKYs in cotton's resistance to . Here, we identified 5, 5, and 10 genes in , , and , respectively, and investigated the expression profiles of all genes in various cotton tissues and in response to hormone treatment or infection. Reverse transcription-quantitative PCR analysis showed that was expressed higher in roots and stems than in other tissues, and up-regulated after inoculation. Knock-down of improved resistance to in both resistant and susceptible cotton cultivars. Comparative analysis of transcriptomes generated from wild-type and stable RNAi (RNA interference) plant with down-regulated showed that genes involved in ethylene (ET) and jasmonic acid (JA) biosynthesis and signaling were significantly upregulated in the RNAi plants. Consistently, the contents of 1-aminocyclopropane-1-carboxylic (ACC), JA, and JA-isoleucine levels were significantly higher in the RNAi plants than in wild-type. Following infection, the levels of ACC and JA decreased in the RNAi plants but still significantly higher (for ACC) than that in wild-type or at the same level (for JA) as in non-infected wild-type plants. Collectively, our results suggested that negatively regulates cotton's resistance to mainly through its effect on ET and JA biosynthesis and signaling pathways.
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2020.00069