Apolipoprotein B and angiotensin-converting enzyme polymorphisms and aerobic interval training: randomized controlled trial in coronary artery disease patients

• Published in: Brazilian journal of medical and biological research 2018-01, Vol.51 (8), p.e6944-e6944
• Main Authors: Tamburus, N Y, Verlengia, R, Kunz, V C, César, M C, Silva, E
• Format: Article
• Language: English
• Subjects: ACE protein; Aerobic capacity; Aerobic exercises; Aerobics; Anaerobic threshold; Anaerobic Threshold - physiology; Angiotensin; Angiotensin converting enzyme; Angiotensin-converting enzyme polymorphism; APOB gene; Apolipoprotein B; Apolipoprotein B polymorphism; Apolipoproteins; Apolipoproteins B - genetics; BIOLOGY; Cardiovascular disease; Cardiovascular diseases; Care and treatment; Case-Control Studies; Coronary artery; Coronary Artery Disease - blood; Coronary Artery Disease - genetics; Coronary Artery Disease - rehabilitation; Coronary heart disease; Coronary vessels; Development and progression; Enzymes; Exercise; Female; Gene Frequency; Gene polymorphism; Genetic aspects; Genetic polymorphisms; Genotype; Health aspects; Heart diseases; Heart Rate - physiology; High-Intensity Interval Training - methods; Humans; Interval training; Lipid metabolism; Lipids; Lipids - blood; Low density lipoprotein; Male; MEDICINE, RESEARCH & EXPERIMENTAL; Middle Aged; Oxygen consumption; Patients; Peptidyl-dipeptidase A; Peptidyl-Dipeptidase A - genetics; Physical fitness; Physical training; Polymerase chain reaction; Polymorphism; Polymorphism, Genetic - genetics; Risk Factors
• ISSN: 0100-879X; 1414-431X; 1414-431X; 1678-4510
• DOI: 10.1590/1414-431x20186944

Physical training has been strongly recommended as a non-pharmacological treatment for coronary artery disease (CAD). Genetic polymorphisms have been studied to understand the biological variability in response to exercise among individuals. This study aimed to verify the possible influence of apolipoprotein B (ApoB: rs1042031 and rs693) and angiotensin-converting enzyme (ACE-ID: rs1799752) genotypes on the lipid profile and functional aerobic capacity, respectively, after an aerobic interval training (AIT) program in patients with CAD and/or cardiovascular risk factors. Sixty-six men were randomized and assigned to trained group (n=32) or control group (n=34). Cardiopulmonary exercise test was performed to determine the ventilatory anaerobic threshold (VAT) from cardiorespiratory variables. The AIT program, at an intensity equivalent to %VAT (70-110%), was conducted three times a week for 16 weeks. ApoB gene polymorphisms (-12669C>T (rs1042031) and -7673G>A (rs693)) were identified by real-time polymerase chain reaction (PCR). I/D polymorphism in the ACE gene (rs1799752) was identified through PCR and fragment size analysis. After 16 weeks, low-density lipoprotein (LDL) levels increased in the trained and control groups with the GA+AA genotype (-7673G>A) of the ApoB gene. Trained groups with ACE-II and ACE-ID genotypes presented an increase in oxygen consumption (VO2VAT) and power output after the AIT program. The presence of the ACE I-allele was associated with increased aerobic functional capacity after the AIT program. Increased LDL levels were observed over time in patients with the -7673G>A polymorphism of the ApoB gene. Trial Registration Information: ClinicalTrials.gov: NCT02313831.