Patients with Coronary Artery Disease Have Lower Levels of Antibody to Heat-Stressed Fibroblast Derived Proteins, versus Normal Subjects

Cellular stress response plays an important role in the pathophysiology of coronary artery disease (CAD). Inhibition of cellular stress may provide a novel clinical approach regarding the diagnosis and treatment of CAD. Fibroblasts constitute 60-70% of cardiac cells and have a crucial role in cardio...

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Bibliographic Details
Published in:Cardiovascular therapeutics 2021-06, Vol.2021, p.1-9
Main Authors: Aghamajidi, Azin, Khameneh, Hesam Babaei, Amirjamshidi, Narges, Jalali, Seyed Farzad, Akhavan-Niaki, Haleh, Khafri, Soraya, Mousavi, Seyedeh Narges, Golpour, Monireh, Mehri, Maryam, AmrollahMostafazadeh
Format: Article
Language:English
Subjects:
Analysis
Antibodies
Atherosclerosis
Cardiac stress tests
Cardiovascular disease
Cell culture
Coronary heart disease
Coronary vessels
Enzyme-linked immunosorbent assay
Ethylenediaminetetraacetic acid
Experiments
Fibroblasts
Flow cytometry
Health aspects
Heat shock proteins
Oxidative stress
Proteins
Tumor necrosis factor-TNF
Viral antibodies
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Summary:Cellular stress response plays an important role in the pathophysiology of coronary artery disease (CAD). Inhibition of cellular stress may provide a novel clinical approach regarding the diagnosis and treatment of CAD. Fibroblasts constitute 60-70% of cardiac cells and have a crucial role in cardiovascular function. Hence, the aim of this study was to show a potential therapeutic application of proteins derived from heat-stressed fibroblast in CAD patients. Fibroblasts were isolated from the foreskin and cultured under heat stress conditions. Surprisingly, 1.06% of the cells exhibited a necrotic death pattern. Furthermore, heat-stressed fibroblasts produced higher level of total proteins than control cells. In SDS-PAGE analysis, a 70 kDa protein band was observed in stressed cell culture supernatants which appeared as two acidic spots with close pI in the two-dimensional electrophoresis. To evaluate the immunogenic properties of fibroblast-derived heat shock proteins (HSPs), the serum immunoglobulin-G (IgG) was measured by ELISA in 50 CAD patients and 50 normal subjects who had been diagnosed through angiography. Interestingly, the level of anti-HSP antibody was significantly higher in non-CAD individuals in comparison with the patient’s group (p
ISSN:1755-5914
1755-5922
DOI:10.1155/2021/5577218