Ex vivo Culture of Adult Mouse Antral Glands

The tri-dimensional culture, initially described by Sato (2009) in order to isolate and characterize epithelial stem cells of the adult small intestine, has been subsequently adapted to many different organs. One of the first examples was the isolation and culture of antral stem cells by Barker (201...

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Bibliographic Details
Published in:Bio-protocol 2017-01, Vol.7 (1), p.e2088-e2088
Main Authors: Vallone, Valeria Fernandez, Leprovots, Morgane, Vassart, Gilbert, Garcia, Marie-Isabelle
Format: Article
Language:English
Subjects:
Biology
Methods
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Summary:The tri-dimensional culture, initially described by Sato (2009) in order to isolate and characterize epithelial stem cells of the adult small intestine, has been subsequently adapted to many different organs. One of the first examples was the isolation and culture of antral stem cells by Barker (2010), who efficiently generated organoids that recapitulate the mature pyloric epithelium . This approach is suitable and promising to study gastric function in homeostasis as well as in disease. We have adapted Barker's protocol to compare homeostatic and regenerating tissues and here, we meticulously describe, step by step, the isolation and culture of antral glands as well as the isolation of single cells from antral glands that might be useful for culture after cell sorting as an example (Fernandez Vallone , 2016 ).
ISSN:2331-8325
2331-8325
DOI:10.21769/BioProtoc.2088