Evaluation of Poly(Lactic-co-glycolic) Acid Alone or in Combination with Hydroxyapatite on Human-Periosteal Cells Bone Differentiation and in Sinus Lift Treatment

• Published in: Molecules (Basel, Switzerland) Switzerland), 2017-12, Vol.22 (12), p.2109
• Main Authors: Ceccarelli, Gabriele, Presta, Rossella, Lupi, Saturnino Marco, Giarratana, Nefele, Bloise, Nora, Benedetti, Laura, Cusella De Angelis, Maria Gabriella, Rodriguez Y Baena, Ruggero
• Format: Article
• Language: English
• Subjects: Acids; Animals; Biocompatibility; Biomedical materials; Bone healing; Bone Regeneration; Calcification, Physiologic - drug effects; Cell Adhesion; Cell Differentiation - drug effects; Cell Proliferation; Cell Survival; Cells, Cultured; Collagen; Collagen - chemistry; Collagen - pharmacology; Dental materials; Dentistry; Durapatite - chemistry; Durapatite - pharmacology; Gene expression; Healing; Horses; Humans; Hydroxyapatite; Lift; Mineralization; Oral Surgical Procedures; Osteoconduction; osteoconductivity; Osteogenesis; Paranasal Sinuses - surgery; Periosteum; Periosteum - cytology; Periosteum - drug effects; periosteum-derived stem cells; poly(lactic-co-glycolic) acid-based materials; Polylactic Acid-Polyglycolic Acid Copolymer - chemistry; Polylactic Acid-Polyglycolic Acid Copolymer - pharmacology; Polylactide-co-glycolide; Scaffolds; Sinus; Sinuses; Soft tissues; Stem cells; Surgery; Surgical implants; Tissue engineering; Tissue Engineering - methods; Tissue Scaffolds
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules22122109

Most recent advances in tissue engineering in the fields of oral surgery and dentistry have aimed to restore hard and soft tissues. Further improvement of these therapies may involve more biological approaches and the use of dental tissue stem cells in combination with inorganic/organic scaffolds. In this study, we analyzed the osteoconductivity of two different inorganic scaffolds based on poly (lactic-co-glycolic) acid alone (PLGA-Fisiograft) or in combination with hydroxyapatite (PLGA/HA-Alos) in comparison with an organic material based on equine collagen (PARASORB Sombrero) both in vitro and in vivo. We developed a simple in vitro model in which periosteum-derived stem cells were grown in contact with chips of these scaffolds to mimic bone mineralization. The viability of cells and material osteoconductivity were evaluated by osteogenic gene expression and histological analyses at different time points. In addition, the capacity of scaffolds to improve bone healing in sinus lift was examined. Our results demonstrated that the osteoconductivity of PLGA/HA-Alos and the efficacy of scaffolds in promoting bone healing in the sinus lift were increased. Thus, new clinical approaches in sinus lift follow-up should be considered to elucidate the clinical potential of these two PLGA-based materials in dentistry.