Genome Analysis of a Novel Tembusu Virus in Taiwan

We identified and isolated a novel Tembusu virus (TMUV) strain TP1906 (TMUV-TP1906) from a mosquito pool collected from the northern part of Taiwan in 2019. The TMUV-TP1906 genome is a 10,990-nucleotide-long, positive-sense, single-stranded RNA, consisting of a single open reading frame (ORF) encodi...

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Published in:Viruses 2020-05, Vol.12 (5), p.567
Main Authors: Peng, Shih-Huan, Su, Chien-Ling, Chang, Mei-Chun, Hu, Huai-Chin, Yang, Su-Lin, Shu, Pei-Yun
Format: Article
Language:English
Subjects:
3' Untranslated regions
Animals
Base Sequence
Cell culture
Cell Line
Chickens
Chlorocebus aethiops
Culicidae - virology
Deoxyribonucleic acid
DNA
Encephalitis
Fibroblasts
Flavivirus - classification
Flavivirus - genetics
Flavivirus - growth & development
Flavivirus - isolation & purification
flavivirus 3′-UTR variable region
Flavivirus Infections - veterinary
Flavivirus Infections - virology
Flaviviruses
Genetic aspects
Genome, Viral
Genomes
Host range
Host Specificity
Kinetics
Mosquitoes
Nucleotide sequence
Open Reading Frames
Phylogeny
Polyproteins - genetics
Poultry Diseases - virology
Ribonucleic acid
RNA
Sitiawan virus
Taiwan
Tembusu virus
Variable region
Vero Cells
Virulence
Viruses
Wetlands
Whole Genome Sequencing
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creator Peng, Shih-Huan
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Shu, Pei-Yun
description We identified and isolated a novel Tembusu virus (TMUV) strain TP1906 (TMUV-TP1906) from a mosquito pool collected from the northern part of Taiwan in 2019. The TMUV-TP1906 genome is a 10,990-nucleotide-long, positive-sense, single-stranded RNA, consisting of a single open reading frame (ORF) encoding a polyprotein of 3425 amino acids, with 5' and 3' untranslated regions (UTRs) of 94 and 618 nucleotides, respectively. The nucleotide sequence of the TMUV-TP1906 of ORF exhibited 93.71% and 91.27% similarity with Sitiawan virus (STWV) and the TMUV prototype strain MM1775, respectively. The 3'-UTR variable region of TMUV-TP1906 showed nucleotide sequence divergence with other TMUV strains. Phylogenetic analysis of the complete ORF and polyprotein sequences revealed that TMUV-TP1906 is most closely related to STWV which causes encephalitis and retarded growth in chickens. We found that the TMUV-TP1906 caused a cytopathic effect (CPE) in the DF-1 chicken fibroblast cell line, while no apparent CPE was observed in Vero and C6/36 cells. In this study, we first identified and isolated a novel TMUV strain in Taiwan. In addition, to our knowledge, it is the first time that the TMUV strain was isolated from the mosquitoes. Further study is warranted to investigate the host range and virulence of TMUV-TP1906.
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subjects 3' Untranslated regions
Animals
Base Sequence
Cell culture
Cell Line
Chickens
Chlorocebus aethiops
Culicidae - virology
Deoxyribonucleic acid
DNA
Encephalitis
Fibroblasts
Flavivirus - classification
Flavivirus - genetics
Flavivirus - growth & development
Flavivirus - isolation & purification
flavivirus 3′-UTR variable region
Flavivirus Infections - veterinary
Flavivirus Infections - virology
Flaviviruses
Genetic aspects
Genome, Viral
Genomes
Host range
Host Specificity
Kinetics
Mosquitoes
Nucleotide sequence
Open Reading Frames
Phylogeny
Polyproteins - genetics
Poultry Diseases - virology
Ribonucleic acid
RNA
Sitiawan virus
Taiwan
Tembusu virus
Variable region
Vero Cells
Virulence
Viruses
Wetlands
Whole Genome Sequencing
title Genome Analysis of a Novel Tembusu Virus in Taiwan
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