β-Sitosterol Reduces the Expression of Chemotactic Cytokine Genes in Cystic Fibrosis Bronchial Epithelial Cells

Extracts from L. seeds, which are widely used as anti-inflammatory remedies in traditional medicine of Northern Africa, were able to inhibit the expression of the pro-inflammatory neutrophil chemokine Interleukin (IL)-8 in Cystic Fibrosis (CF) bronchial epithelial IB3-1 cells exposed to the Gram-neg...

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Published in:Frontiers in pharmacology 2017-05, Vol.8, p.236-236
Main Authors: Lampronti, Ilaria, Dechecchi, Maria C, Rimessi, Alessandro, Bezzerri, Valentino, Nicolis, Elena, Guerrini, Alessandra, Tacchini, Massimo, Tamanini, Anna, Munari, Silvia, D'Aversa, Elisabetta, Santangelo, Alessandra, Lippi, Giuseppe, Sacchetti, Gianni, Pinton, Paolo, Gambari, Roberto, Agostini, Maddalena, Cabrini, Giulio
Format: Article
Language:English
Subjects:
cystic fibrosis
cytokines
inflammation
interleukin-8
Nigella arvensis
Pharmacology
β-sitosterol
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Summary:Extracts from L. seeds, which are widely used as anti-inflammatory remedies in traditional medicine of Northern Africa, were able to inhibit the expression of the pro-inflammatory neutrophil chemokine Interleukin (IL)-8 in Cystic Fibrosis (CF) bronchial epithelial IB3-1 cells exposed to the Gram-negative bacterium . The chemical composition of the extracts led to the identification of three major components, β-sitosterol, stigmasterol, and campesterol, which are the most abundant phytosterols, cholesterol-like molecules, usually found in plants. β-sitosterol (BSS) was the only compound that significantly reproduced the inhibition of the -dependent expression of IL-8 at nanomolar concentrations. BSS was tested in CF airway epithelial CuFi-1 cells infected with . BSS (100 nM), showed a significant and consistent inhibitory activity on expression of the -stimulated expression chemokines IL-8, GRO-α GRO-β, which play a pivotal role in the recruitment of neutrophils in CF inflamed lungs. Preliminary mechanistic analysis showed that BSS partially inhibits the -dependent activation of Protein Kinase C isoform alpha, which is known to be involved in the transmembrane signaling activating IL-8 gene expression in bronchial epithelial cells. These data indicate BSS as a promising molecule to control excessive lung inflammation in CF patients.
ISSN:1663-9812
1663-9812
DOI:10.3389/fphar.2017.00236