Differential expression of drug resistance genes in CD146 positive dental pulp derived stem cells and CD146 negative fibroblasts

Introduction The stem cell portion of the dental pulp derived cultures (DPSCs) showed a higher resistance to cytotoxic effect of restorative dental materials compared to pulpal fibroblasts (DPFs). Here, we aimed to compare the expression of some drug resistant genes between these cells. Methods and...

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Bibliographic Details
Published in:Clinical and experimental dental research 2020-08, Vol.6 (4), p.448-456
Main Authors: Tavangar, Maryam S., Attar, Armin, Razmkhah, Mahboobeh, Hosseini, Seyed‐Mojtaba, Hosseini, Ahmad, Monabati, Ahmad, Shafiei, Fereshteh
Format: Article
Language:English
Subjects:
ABC transporter
Adipocytes
Antibodies
Antigens
Bone marrow
CD146
Cytotoxicity
Dental pulp
Dentistry
Drug resistance
fibroblast
Fibroblasts
Flow cytometry
Genes
Growth factors
Original
Polymerase chain reaction
stem cell
Stem cells
Thermal cycling
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Summary:Introduction The stem cell portion of the dental pulp derived cultures (DPSCs) showed a higher resistance to cytotoxic effect of restorative dental materials compared to pulpal fibroblasts (DPFs). Here, we aimed to compare the expression of some drug resistant genes between these cells. Methods and materials To separate DPSCs from DPFs, we used magnetic cell sorting technique based on CD146 expression. To assess the stem cell properties, the positive and negative portions underwent colony forming assays and were induced to be differentiated into the adipocytes, osteoblasts, hepatocytes, and neural cells. Cell surface antigen panels were checked using immune fluorescence and flow‐cytometry techniques. The mRNA expression of 14 ABC transporters including ABCA2, ABCB1, ABCB11, ABCC1, ABCC2, ABCC3, ABCC4, ABCC5‐2, ABCC5‐4,ABCC5‐13, ABCC6, ABCC10, ABCC11, and ABCG2 genes was assessed, using quantitative RT‐PCR technique. Results Only the CD146 positive portion could be differentiated into the desired fates, and they formed higher colonies (16.7 ± 3.32 vs. 1.7 ± 1.67, p 
ISSN:2057-4347
2057-4347
DOI:10.1002/cre2.297