Differential expression of drug resistance genes in CD146 positive dental pulp derived stem cells and CD146 negative fibroblasts

Introduction The stem cell portion of the dental pulp derived cultures (DPSCs) showed a higher resistance to cytotoxic effect of restorative dental materials compared to pulpal fibroblasts (DPFs). Here, we aimed to compare the expression of some drug resistant genes between these cells. Methods and...

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Published in:Clinical and experimental dental research 2020-08, Vol.6 (4), p.448-456
Main Authors: Tavangar, Maryam S., Attar, Armin, Razmkhah, Mahboobeh, Hosseini, Seyed‐Mojtaba, Hosseini, Ahmad, Monabati, Ahmad, Shafiei, Fereshteh
Format: Article
Language:English
Subjects:
ABC transporter
Adipocytes
Antibodies
Antigens
Bone marrow
CD146
Cytotoxicity
Dental pulp
Dentistry
Drug resistance
fibroblast
Fibroblasts
Flow cytometry
Genes
Growth factors
Original
Polymerase chain reaction
stem cell
Stem cells
Thermal cycling
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container_title Clinical and experimental dental research
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creator Tavangar, Maryam S.
Attar, Armin
Razmkhah, Mahboobeh
Hosseini, Seyed‐Mojtaba
Hosseini, Ahmad
Monabati, Ahmad
Shafiei, Fereshteh
description Introduction The stem cell portion of the dental pulp derived cultures (DPSCs) showed a higher resistance to cytotoxic effect of restorative dental materials compared to pulpal fibroblasts (DPFs). Here, we aimed to compare the expression of some drug resistant genes between these cells. Methods and materials To separate DPSCs from DPFs, we used magnetic cell sorting technique based on CD146 expression. To assess the stem cell properties, the positive and negative portions underwent colony forming assays and were induced to be differentiated into the adipocytes, osteoblasts, hepatocytes, and neural cells. Cell surface antigen panels were checked using immune fluorescence and flow‐cytometry techniques. The mRNA expression of 14 ABC transporters including ABCA2, ABCB1, ABCB11, ABCC1, ABCC2, ABCC3, ABCC4, ABCC5‐2, ABCC5‐4,ABCC5‐13, ABCC6, ABCC10, ABCC11, and ABCG2 genes was assessed, using quantitative RT‐PCR technique. Results Only the CD146 positive portion could be differentiated into the desired fates, and they formed higher colonies (16.7 ± 3.32 vs. 1.7 ± 1.67, p 
doi_str_mv 10.1002/cre2.297
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Here, we aimed to compare the expression of some drug resistant genes between these cells. Methods and materials To separate DPSCs from DPFs, we used magnetic cell sorting technique based on CD146 expression. To assess the stem cell properties, the positive and negative portions underwent colony forming assays and were induced to be differentiated into the adipocytes, osteoblasts, hepatocytes, and neural cells. Cell surface antigen panels were checked using immune fluorescence and flow‐cytometry techniques. The mRNA expression of 14 ABC transporters including ABCA2, ABCB1, ABCB11, ABCC1, ABCC2, ABCC3, ABCC4, ABCC5‐2, ABCC5‐4,ABCC5‐13, ABCC6, ABCC10, ABCC11, and ABCG2 genes was assessed, using quantitative RT‐PCR technique. Results Only the CD146 positive portion could be differentiated into the desired fates, and they formed higher colonies (16.7 ± 3.32 vs. 1.7 ± 1.67, p &lt; .001). The cell surface antigen panels were the same, except for CD146 and STRO‐1 markers which were expressed only in the positive portion. Among the ABC transporter genes studied, the positive portion showed a higher expression (approximately two‐fold) of ABCA2, ABCC5‐13, and ABCC5‐2 genes. Conclusion Dental pulp stem cells which can be separated from dental pulp fibroblasts based on CD146 expression, express higher levels of some drug resistance genes which probably accounts for their features of more resistance to cytotoxic effects of some dental materials. This needs to be more validated in future.</description><identifier>ISSN: 2057-4347</identifier><identifier>EISSN: 2057-4347</identifier><identifier>DOI: 10.1002/cre2.297</identifier><identifier>PMID: 32378809</identifier><language>eng</language><publisher>United States: John Wiley &amp; Sons, Inc</publisher><subject>ABC transporter ; Adipocytes ; Antibodies ; Antigens ; Bone marrow ; CD146 ; Cytotoxicity ; Dental pulp ; Dentistry ; Drug resistance ; fibroblast ; Fibroblasts ; Flow cytometry ; Genes ; Growth factors ; Original ; Polymerase chain reaction ; stem cell ; Stem cells ; Thermal cycling</subject><ispartof>Clinical and experimental dental research, 2020-08, Vol.6 (4), p.448-456</ispartof><rights>2020 The Authors. published by John Wiley &amp; Sons Ltd</rights><rights>2020 The Authors. Clinical and Experimental Dental Research published by John Wiley &amp; Sons Ltd.</rights><rights>2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5047-5d2af06b7d5fa2b01088cc79e096586787974ba3fa682e6fd0e889b5e309bafc3</citedby><cites>FETCH-LOGICAL-c5047-5d2af06b7d5fa2b01088cc79e096586787974ba3fa682e6fd0e889b5e309bafc3</cites><orcidid>0000-0002-4133-4870</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2437799466/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2437799466?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,11562,25753,27924,27925,37012,37013,44590,46052,46476,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32378809$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tavangar, Maryam S.</creatorcontrib><creatorcontrib>Attar, Armin</creatorcontrib><creatorcontrib>Razmkhah, Mahboobeh</creatorcontrib><creatorcontrib>Hosseini, Seyed‐Mojtaba</creatorcontrib><creatorcontrib>Hosseini, Ahmad</creatorcontrib><creatorcontrib>Monabati, Ahmad</creatorcontrib><creatorcontrib>Shafiei, Fereshteh</creatorcontrib><title>Differential expression of drug resistance genes in CD146 positive dental pulp derived stem cells and CD146 negative fibroblasts</title><title>Clinical and experimental dental research</title><addtitle>Clin Exp Dent Res</addtitle><description>Introduction The stem cell portion of the dental pulp derived cultures (DPSCs) showed a higher resistance to cytotoxic effect of restorative dental materials compared to pulpal fibroblasts (DPFs). Here, we aimed to compare the expression of some drug resistant genes between these cells. Methods and materials To separate DPSCs from DPFs, we used magnetic cell sorting technique based on CD146 expression. To assess the stem cell properties, the positive and negative portions underwent colony forming assays and were induced to be differentiated into the adipocytes, osteoblasts, hepatocytes, and neural cells. Cell surface antigen panels were checked using immune fluorescence and flow‐cytometry techniques. The mRNA expression of 14 ABC transporters including ABCA2, ABCB1, ABCB11, ABCC1, ABCC2, ABCC3, ABCC4, ABCC5‐2, ABCC5‐4,ABCC5‐13, ABCC6, ABCC10, ABCC11, and ABCG2 genes was assessed, using quantitative RT‐PCR technique. Results Only the CD146 positive portion could be differentiated into the desired fates, and they formed higher colonies (16.7 ± 3.32 vs. 1.7 ± 1.67, p &lt; .001). The cell surface antigen panels were the same, except for CD146 and STRO‐1 markers which were expressed only in the positive portion. Among the ABC transporter genes studied, the positive portion showed a higher expression (approximately two‐fold) of ABCA2, ABCC5‐13, and ABCC5‐2 genes. Conclusion Dental pulp stem cells which can be separated from dental pulp fibroblasts based on CD146 expression, express higher levels of some drug resistance genes which probably accounts for their features of more resistance to cytotoxic effects of some dental materials. 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Here, we aimed to compare the expression of some drug resistant genes between these cells. Methods and materials To separate DPSCs from DPFs, we used magnetic cell sorting technique based on CD146 expression. To assess the stem cell properties, the positive and negative portions underwent colony forming assays and were induced to be differentiated into the adipocytes, osteoblasts, hepatocytes, and neural cells. Cell surface antigen panels were checked using immune fluorescence and flow‐cytometry techniques. The mRNA expression of 14 ABC transporters including ABCA2, ABCB1, ABCB11, ABCC1, ABCC2, ABCC3, ABCC4, ABCC5‐2, ABCC5‐4,ABCC5‐13, ABCC6, ABCC10, ABCC11, and ABCG2 genes was assessed, using quantitative RT‐PCR technique. Results Only the CD146 positive portion could be differentiated into the desired fates, and they formed higher colonies (16.7 ± 3.32 vs. 1.7 ± 1.67, p &lt; .001). The cell surface antigen panels were the same, except for CD146 and STRO‐1 markers which were expressed only in the positive portion. Among the ABC transporter genes studied, the positive portion showed a higher expression (approximately two‐fold) of ABCA2, ABCC5‐13, and ABCC5‐2 genes. Conclusion Dental pulp stem cells which can be separated from dental pulp fibroblasts based on CD146 expression, express higher levels of some drug resistance genes which probably accounts for their features of more resistance to cytotoxic effects of some dental materials. This needs to be more validated in future.</abstract><cop>United States</cop><pub>John Wiley &amp; Sons, Inc</pub><pmid>32378809</pmid><doi>10.1002/cre2.297</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-4133-4870</orcidid><oa>free_for_read</oa></addata></record>
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subjects ABC transporter
Adipocytes
Antibodies
Antigens
Bone marrow
CD146
Cytotoxicity
Dental pulp
Dentistry
Drug resistance
fibroblast
Fibroblasts
Flow cytometry
Genes
Growth factors
Original
Polymerase chain reaction
stem cell
Stem cells
Thermal cycling
title Differential expression of drug resistance genes in CD146 positive dental pulp derived stem cells and CD146 negative fibroblasts
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