Simultaneous Promotion of Salt Tolerance and Phenolic Acid Biosynthesis in Salvia miltiorrhiza via Overexpression of Arabidopsis MYB12

Transcription factors play crucial roles in regulating plant abiotic stress responses and physiological metabolic processes, which can be used for plant molecular breeding. In this study, an R2R3-MYB transcription factor gene, AtMYB12, was isolated from Arabidopsis thaliana and introduced into Salvi...

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Published in:International journal of molecular sciences 2023-11, Vol.24 (21), p.15506
Main Authors: Li, Tianyu, Zhang, Shuangshuang, Li, Yidan, Zhang, Lipeng, Song, Wenqin, Chen, Chengbin, Ruan, Weibin
Format: Article
Language:English
Subjects:
Abiotic stress
Acids
Antioxidants
AtMYB12
Biosynthesis
Chlorophyll
Enzymes
Genes
Leaves
Metabolism
phenolic acid
Physiology
Plant growth
Proteins
Reactive oxygen species
Salinity
salt tolerance
Salvia miltiorrhiza
Signal transduction
Transcription factors
Transgenic plants
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Summary:Transcription factors play crucial roles in regulating plant abiotic stress responses and physiological metabolic processes, which can be used for plant molecular breeding. In this study, an R2R3-MYB transcription factor gene, AtMYB12, was isolated from Arabidopsis thaliana and introduced into Salvia miltiorrhiza under the regulation of the CaMV35S promoter. The ectopic expression of AtMYB12 resulted in improved salt tolerance in S. miltiorrhiza; transgenic plants showed a more resistant phenotype under high-salinity conditions. Physiological experiments showed that transgenic plants exhibited higher chlorophyll contents, and decreased electrolyte leakage and O2− and H2O2 accumulation when subjected to salt stress. Moreover, the activity of reactive oxygen species (ROS)-scavenging enzymes was enhanced in S. miltiorrhiza via the overexpression of AtMYB12, and transgenic plants showed higher superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) activities compared with those of the wild type (WT) under salt stress, coupled with lower malondialdehyde (MDA) levels. In addition, the amount of salvianolic acid B was significantly elevated in all AtMYB12 transgenic hair roots and transgenic plants, and qRT-PCR analysis revealed that most genes in the phenolic acid biosynthetic pathway were up-regulated. In conclusion, these results demonstrated that AtMYB12 can significantly improve the resistance of plants to salt stress and promote the biosynthesis of phenolic acids by regulating genes involved in the biosynthetic pathway.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms242115506