Cytokinin Type-B Response Regulators Promote Bulbil Initiation in Lilium lancifolium

The bulbil is an important vegetative reproductive organ in triploid whose development is promoted by cytokinins. Type-B response regulators (RRs) are critical regulators that mediate primary cytokinin responses and promote cytokinin-induced gene expression. However, the function of cytokinin type-B...

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Bibliographic Details
Published in:International journal of molecular sciences 2021-03, Vol.22 (7), p.3320
Main Authors: He, Guoren, Yang, Panpan, Cao, Yuwei, Tang, Yuchao, Wang, Ling, Song, Meng, Wang, Jing, Xu, Leifeng, Ming, Jun
Format: Article
Language:English
Subjects:
bulbil formation
Cloning
cytokinin
Cytokinins
Deoxyribonucleic acid
DNA
Domains
Functional analysis
Gene expression
Homology
Kinases
Lilium lancifolium
Localization
Meristems
N-Terminus
Phylogenetics
Proteins
Reproductive organs
Signal transduction
Transcription factors
type-B response regulators
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Summary:The bulbil is an important vegetative reproductive organ in triploid whose development is promoted by cytokinins. Type-B response regulators (RRs) are critical regulators that mediate primary cytokinin responses and promote cytokinin-induced gene expression. However, the function of cytokinin type-B Arabidopsis RRs (ARRs) in regulating bulbil formation is unclear. In this study, we identified five type-B s, , , , and , in for the first time. The five s encode proteins of 715, 675, 573, 582 and 647 amino acids. All of the regulators belong to the B-I subfamily, whose members typically contain a conserved CheY-homologous receiver (REC) domain and an Myb DNA-binding (MYB) domain at the N-terminus. As transcription factors, all five type-B LlRRs localize at the nucleus and are widely expressed in plant tissues, especially during axillary meristem (AM) formation. Functional analysis showed that type-B s are involved in bulbil formation in a functionally redundant manner and can activate expression. In summary, our study elucidates the process by which cytokinins regulate bulbil initiation in through type-B LlRRs and lays a foundation for research on the molecular mechanism of bulbil formation in the lily.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22073320