Analysis of miRNAs in milk of four livestock species

• Published in: BMC genomics 2024-09, Vol.25 (1), p.859-15, Article 859
• Main Authors: Cendron, Filippo, Rosani, Umberto, Franzoi, Marco, Boselli, Carlo, Maggi, Flavio, De Marchi, Massimo, Penasa, Mauro
• Format: Article
• Language: English
• Subjects: Analysis; Animals; Annotations; Biomarkers; Body fluids; Bubalus bubalis; Buffalo; Capra hircus; Composition; Donkey; Donkeys; Environmental factors; Equus asinus; Female; Functionals; Gene expression; Gene Expression Profiling; Gene sequencing; Genes; Genetic analysis; Genetic aspects; Genetic transcription; Genomes; Goat; Goats; Goats - genetics; High-Throughput Nucleotide Sequencing; Immune response; Immune system; Kinases; Lactation; Livestock; Livestock - genetics; Measurement; Meat production; MicroRNA; MicroRNAs; MicroRNAs - genetics; Milk; Milk - metabolism; Milk production; miRNA; ncRNAs; Neonates; Next-generation sequencing; Nutrients; Ovis aries; Physiological aspects; Physiology; Post-transcription; Proteins; Ribonucleic acid; RNA; Sequence Analysis, RNA; Sheep; Sheep - genetics; Species Specificity; Target detection; Water buffalo; Wildlife conservation
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-024-10783-4

Milk is essential for mammalian nutrition because it provides vital nutrients for growth and development. Milk composition, which is influenced by genetic and environmental factors, supports lactation, a complex process crucial for milk production and quality. Recent research has focused on noncoding RNAs, particularly microRNAs (miRNAs), which are present in body fluids and regulate gene expression post-transcriptionally. This study comprehensively characterizes miRNAs in milk of four livestock species, namely Bubalus bubalis, Capra hircus, Equus asinus, and Ovis aries and identifies potential target genes. High-throughput sequencing of milk RNA resulted in distinct read counts across species: B. bubalis (8,790,441 reads), C. hircus (12,976,275 reads), E. asinus (9,385,067 reads), and O. aries (7,295,297 reads). E. asinus had the highest RNA mapping rate (94.6%) and O. aries the lowest (84.8%). A substantially greater proportion of miRNAs over other small RNAs was observed for the donkey milk sample (7.74%) compared to buffalo (0.87%), goat (1.57%), and sheep (1.12%). Shared miRNAs, which included miR-200a, miR-200b, miR-200c, and miR-23a among others, showed varying expression levels across species, confirmed by qPCR analysis. Functional annotation of predicted miRNA target genes highlighted diverse roles, with an enrichment in functions linked to metabolism and immunity. Pathway analysis identified immune response pathways as significant, with several miRNAs targeting specific genes across species, suggesting their regulatory function in milk. Both conserved and species-specific miRNAs were detected in milk of the investigated species. The identified target genes of these miRNAs have important roles in neonatal development, adaptation, growth, and immune response. Furthermore, they influence milk and meat production traits in livestock.