Loliolide from Artemisia princeps Suppresses Adipogenesis in Human Bone Marrow-Derived Mesenchymal Stromal Cells via Activation of AMPK and Wnt/β-catenin Pathways

Regulating the adipogenic differentiation mechanism is a valid and promising mechanism to battle obesity. Natural products, especially phytochemicals as nutraceuticals, are important lead molecules with significant activities against obesity. Loliolide is a monoterpenoid hydroxyl lactone found in ma...

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Bibliographic Details
Published in:Applied sciences 2021-06, Vol.11 (12), p.5435
Main Authors: Oh, Jung Hwan, Karadeniz, Fatih, Jang, Mi-Soon, Kim, Hojun, Seo, Youngwan, Kong, Chang-Suk
Format: Article
Language:English
Subjects:
Adipocytes
Adipogenesis
AMPK
Artemisia princeps
Bone marrow
Cell activation
Diabetes
Differentiation
Functional foods & nutraceuticals
hBM-MSC
Immunoblotting
Lead
Lead compounds
Lipids
loliolide
MAP kinase
Medical research
Mesenchyme
Natural products
Obesity
Osteoporosis
Phosphorylation
Proteins
Regulation
Stains & staining
Stem cells
Stromal cells
Transcription factors
Translocation
Wnt protein
β-Catenin
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Summary:Regulating the adipogenic differentiation mechanism is a valid and promising mechanism to battle obesity. Natural products, especially phytochemicals as nutraceuticals, are important lead molecules with significant activities against obesity. Loliolide is a monoterpenoid hydroxyl lactone found in many dietary plants. The effect of loliolide on adipogenic differentiation is yet to be determined. Therefore, the present study aimed to evaluate its anti-adipogenic potential using human bone marrow-derived mesenchymal stromal cells (hBM-MSCs) and assess its mechanism of action. Adipo-induced hBM-MSCs were treated with or without loliolide and their adipogenic characteristics were examined. Loliolide treatment decreased the lipid accumulation and expression of adipogenic transcription factors, PPARγ, C/EBPα, and SREBP1c. Adipo-induced hBM-MSCs also displayed increased AMPK phosphorylation and suppressed MAPK activation following loliolide treatment according to immunoblotting results. Importantly, loliolide could stimulate Wnt10b expression and active β-catenin translocation to exert PPARγ-linked adipogenesis suppression. In conclusion, loliolide was suggested to be a potential anti-adipogenic agent which may be utilized as a lead compound for obesity treatment or prevention.
ISSN:2076-3417
2076-3417
DOI:10.3390/app11125435