Inhibition of Candida albicans Biofilm Formation and Attenuation of Its Virulence by Liriope muscari

(1) Background: Although accounts for the majority of fungal infections, therapeutic options are limited and require alternative antifungal agents with new targets; (2) Methods: A biofilm formation assay with RPMI1640 medium was performed with extract. A combination antifungal assay, dimorphic trans...

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Bibliographic Details
Published in:Antibiotics (Basel) 2024-05, Vol.13 (5), p.434
Main Authors: Lee, Jeonghoon, Song, Hyunchan, Kim, Kiyoung
Format: Article
Language:English
Subjects:
adherence assay
Antibiotics
antifungal agent
Antifungal agents
Assaying
biofilm formation
Biofilms
Candida
Candida albicans
Candidiasis
dimorphic transition
Extracellular matrix
Fungicides
Gene expression
Genes
Infections
Kinases
Liriope muscari
MAP kinase
Miconazole
Pathogenesis
Protein kinase A
Virulence
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Summary:(1) Background: Although accounts for the majority of fungal infections, therapeutic options are limited and require alternative antifungal agents with new targets; (2) Methods: A biofilm formation assay with RPMI1640 medium was performed with extract. A combination antifungal assay, dimorphic transition assay, and adhesion assay were performed under the biofilm formation condition to determine the anti-biofilm formation effect. qRT-PCR analysis was accomplished to confirm changes in gene expression; (3) Results: extract significantly reduces biofilm formation by 51.65% at 1.56 μg/mL use and therefore increases susceptibility to miconazole. extract also inhibited the dimorphic transition of Candida; nearly 50% of the transition was inhibited when 1.56 μg/mL of the extract was treated. The extract of inhibited the expression of genes related to hyphal development and extracellular matrix of 34.4% and 36.0%, respectively, as well as genes within the Ras1-cAMP-PKA, Cph2-Tec1, and MAP kinase signaling pathways of 25.58%, 7.1% and 15.8%, respectively, at 1.56 μg/mL of extract treatment; (4) Conclusions: extract significantly reduced Candida biofilm formation, which lead to induced antifungal susceptibility to miconazole. It suggests that extract is a promising anti-biofilm candidate of since the biofilm formation of is an excellent target for candidiasis regulation.
ISSN:2079-6382
2079-6382
DOI:10.3390/antibiotics13050434