Analysis of Expression Pattern of snoRNAs in Human Cells A549 Infected by Influenza A Virus

• Published in: International journal of molecular sciences 2022-11, Vol.23 (22), p.13666
• Main Authors: Zhuravlev, Evgenii, Sergeeva, Mariia, Malanin, Sergey, Amirkhanov, Rinat, Semenov, Dmitriy, Grigoryeva, Tatiana, Komissarov, Andrey, Stepanov, Grigory
• Format: Article
• Language: English
• Subjects: Biosynthesis; Cancer; Gene expression; Genes; Genomes; Genomics; H1N1; Humans; Infections; Influenza; Influenza A; influenza A virus; Influenza A virus - genetics; Influenza A Virus, H1N1 Subtype - genetics; Influenza A Virus, H1N1 Subtype - metabolism; Influenza, Human - genetics; MicroRNAs; ncRNAs; Nucleoli; Pattern analysis; Post-transcription; RNA viruses; RNA, Ribosomal; RNA, Small Nucleolar - genetics; RNA, Small Nucleolar - metabolism; RNA-seq; sdRNAs; Sequence analysis; snoRNA; snoRNAs; Splicing; Viral infections; Viruses
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms232213666

Small nucleolar RNAs (snoRNAs) are a highly expressed class of non-coding RNAs known for their role in guiding post-transcriptional modifications of ribosomal RNAs and small nuclear RNAs. Emerging studies suggest that snoRNAs are also implicated in regulating other vital cellular processes, such as pre-mRNA splicing and 3'-processing of mRNAs, and in the development of cancer and viral infections. There is an emerging body of evidence for specific snoRNA's involvement in the optimal replication of RNA viruses. In order to investigate the expression pattern of snoRNAs during influenza A viral infection, we performed RNA sequencing analysis of the A549 human cell line infected by influenza virus A/Puerto Rico/8/1934 (H1N1). We identified 66 that were upregulated and 55 that were downregulated in response to influenza A virus infection. The increased expression of most C/D-box snoRNAs was associated with elevated levels of 5'- and 3'-short RNAs derived from this snoRNA. Analysis of the poly(A)+ RNA sequencing data indicated that most of the differentially expressed snoRNAs synthesis was not correlated with the corresponding host genes expression. Furthermore, influenza A viral infection led to an imbalance in the expression of genes responsible for C/D small nucleolar ribonucleoprotein particles' biogenesis. In summary, our results indicate that the expression pattern of snoRNAs in A549 cells is significantly altered during influenza A viral infection.