Physiological and Genomic Characterization of Actinotalea subterranea sp. nov. from Oil-Degrading Methanogenic Enrichment and Reclassification of the Family Actinotaleaceae

• Published in: Microorganisms (Basel) 2022-02, Vol.10 (2), p.378
• Main Authors: Semenova, Ekaterina M, Grouzdev, Denis S, Sokolova, Diyana S, Tourova, Tatiyana P, Poltaraus, Andrey B, Potekhina, Natalia V, Shishina, Polina N, Bolshakova, Maria A, Avtukh, Alexander N, Ianutsevich, Elena A, Tereshina, Vera M, Nazina, Tamara N
• Format: Article
• Language: English
• Subjects: Actinotalea subterranea; Actinotaleaceae; Bacteria; Biodegradation; Biomass; Crude oil; Degradation; Deoxyribonucleic acid; DNA; Gene amplification; Gene sequencing; Genera; genome analysis; Genomes; Genomic analysis; Hybridization; Hydrocarbons; Microorganisms; New species; Nucleotide sequence; Nucleotides; Oil; Oil and gas fields; oil field; Oil fields; Oil reservoirs; Physiology; polyphasic taxonomy; Prokaryotes; Reclassification; rRNA 16S; Salinity; Sodium chloride; Taxonomic revision; Taxonomy
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms10020378

The goal of the present work was to determine the diversity of prokaryotes involved in anaerobic oil degradation in oil fields. The composition of the anaerobic oil-degrading methanogenic enrichment obtained from an oil reservoir was determined by 16S rRNA-based survey, and the facultatively anaerobic chemoorganotrophic bacterial strain HO-Ch2 was isolated and studied using polyphasic taxonomy approach and genome sequencing. The strain HO-Ch2 grew optimally at 28 °C, pH 8.0, and 1-2% ( / ) NaCl. The 16S rRNA gene sequence of the strain HO-Ch2 had 98.8% similarity with the sequence of CF5-4 . The genomic DNA G + C content of strain HO-Ch2 was 73.4%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the genome of strain HO-Ch2 and genomes were 79.8-82.0% and 20.5-22.2%, respectively, i.e., below the thresholds for species delineation. Based on the phylogenomic, phenotypic, and chemotaxonomic characterization, we propose strain HO-Ch2 (= VKM Ac-2850 = KCTC 49656 ) as the type strain of a new species within the genus , with the name sp. nov. Based on the phylogenomic analysis of 187 genomes of we propose the taxonomic revision of the genera and and of the family . We also propose the reclassification of as comb. nov., as comb. nov., as comb. nov., and the transfer of the genus to the family of the order .