ECIS Based Electric Fence Method for Measurement of Human Keratinocyte Migration on Different Substrates

Electric Cell-substrate Impedance Sensing (ECIS) is an impedance-based, real-time, and label-free measuring system for monitoring cellular activities in tissue culture. Previously, ECIS wound healing assay has been used to wound cells with high electric current and monitor the subsequent cell migrat...

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Bibliographic Details
Published in:Biosensors (Basel) 2022-05, Vol.12 (5), p.293
Main Authors: Hung, Yu-Han, Chiu, Wei-Chih, Fuh, Shyh-Rong, Lai, Yi-Ting, Tung, Tse-Hua, Huang, Chun-Chung, Lo, Chun-Min
Format: Article
Language:English
Subjects:
Adhesion
Arrays
Automation
Cell Adhesion
Cell adhesion & migration
Cell culture
Cell migration
Cell Movement
Coated electrodes
Coatings
Collagen
ECIS
Electric cells
electric fence (EF)
Electric Impedance
Electrodes
Humans
Impedance
Keratinocytes
Lysine
Morphology
Poly-L-lysine
Substrates
Surface charge
Time measurement
Time series
Tissue culture
Wound healing
wound healing assays
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Summary:Electric Cell-substrate Impedance Sensing (ECIS) is an impedance-based, real-time, and label-free measuring system for monitoring cellular activities in tissue culture. Previously, ECIS wound healing assay has been used to wound cells with high electric current and monitor the subsequent cell migration. In this study, we applied ECIS electric fence (EF) method, an alternative to electrical wounding, to assess the effects of different surface coatings on human keratinocyte (HaCaT) migration. The EF prevents inoculated cells from attaching or migrating to the fenced electrode surface while maintaining the integrity of the surface coating. After the EF is turned off, cells migrate into the cell-free area, and the increase in measured impedance is monitored. We cultured HaCaT cells on gold electrodes without coating or coated with poly-L-lysin (PLL), poly-D-lysine (PDL), or type-I collagen. We quantified migration rates according to the different slopes in the impedance time series. It was observed that either poly-L-lysine (PLL) or poly-D-lysine (PDL) limits cell adhesion and migration rates. Furthermore, the surface charge of the coated substrate in the culture condition positively correlates with the cell adhesion and migration process. Our results indicate that the EF method is useful for determining cell migration rates on specific surface coatings.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios12050293