Enhanced Adhesion of Fish Ovarian Germline Stem Cells on Solid Surfaces by Mussel-Inspired Polymer Coating

Development of advanced cell culture methods has gained increasing attention because it allows for efficient genetic engineering and precise regulation of animal reproduction on a cellular basis. Numerous studies have attempted to develop an advanced cell culture method. Previous studies have altere...

Full description

Saved in:
Bibliographic Details
Published in:Marine drugs 2019-01, Vol.17 (1), p.11
Main Authors: Jeong, Yeonwoo, Ryu, Jun Hyung, Nam, Yoon Kwon, Gong, Seung Pyo, Kang, Sung Min
Format: Article
Language:English
Subjects:
Adhesion
Animals
Bivalvia - chemistry
Cell Adhesion
Cell adhesion & migration
Cell culture
Cell Culture Techniques - methods
Cell lines
Cells, Cultured
Coating effects
Coatings
Contact angle
Culture media
Dopamine
Embryo cells
Embryos
enhanced adhesion
Female
Fish
Fisheries
Genetic engineering
Indoles - chemistry
Laboratory animals
Life sciences
Lysine
Mammalian cells
Mollusks
mussel
Oogonial Stem Cells
Oryzias
ovarian germline stem cell
Ovaries
Photoelectron Spectroscopy
Poly-L-lysine
polydopamine
Polylysine - chemistry
polymer coating
Polymer coatings
Polymers
Polymers - chemistry
Solid surfaces
Stem cell transplantation
Stem cells
Surface Properties
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Development of advanced cell culture methods has gained increasing attention because it allows for efficient genetic engineering and precise regulation of animal reproduction on a cellular basis. Numerous studies have attempted to develop an advanced cell culture method. Previous studies have altered cell culture media and pretreated culture plates with functional molecules. Among them, a mussel-inspired polymer coating has been extensively utilized owing to its wide applicability. For instance, adhesion of human embryonic stem cells and neuronal cells on solid surfaces has been improved. Despite the excellent capability of the mussel-inspired polymer coating, most studies have primarily focused on mammalian cells. However, the efficacy of these coatings on the adhesion of other cell lines is yet unclear. This study aimed to assess the potential of the mussel-inspired polymer coating in the regulation of the adhesion of fish ovarian germline stem cells on solid surfaces. Solid surfaces were coated by polydopamine and poly-L-lysine, and the effect of the coatings on cellular behaviors was investigated.
ISSN:1660-3397
1660-3397
DOI:10.3390/md17010011