Antidepressant, anti-inflammatory, and antioxidant effects of electroacupuncture through sonic hedgehog-signaling pathway in a rat model of poststroke depression

• Published in: Neuropsychiatric disease and treatment 2019-05, Vol.15, p.1403-1411
• Main Authors: Cai, Wa, Ma, Wen, Wang, Guan-Tao, Li, Yi-Jing, Shen, Wei-Dong
• Format: Article
• Language: English
• Subjects: 5-HT; Acupuncture; Anti-inflammatory agents; Antidepressants; Antioxidants; Antioxidants (Nutrients); Behavior; Carotid arteries; Cerebral blood flow; Chinese medicine; Depression (Mood disorder); Drug therapy; Electroacupuncture; Enzyme-linked immunosorbent assay; Experiments; Hedgehog protein; Hippocampus; Inflammation; Interleukin 1; Interleukin 6; Ischemia; Laboratory animals; Locomotor activity; Malondialdehyde; Medical research; Medical supplies; Mental depression; Neurogenesis; Original Research; Oxidative stress; Polyclonal antibodies; post stroke depression; Proteins; Signal transduction; sonic hedgehog signaling pathway; Stroke; Sucrose; Tumor necrosis factor; Tumor necrosis factor-α; Veins & arteries; Western blotting
• ISSN: 1176-6328; 1178-2021; 1178-2021
• DOI: 10.2147/NDT.S205033

Poststroke depression (PSD) is the most frequent psychological sequela after stroke. Electroacupuncture (EA) treatment is effective for PSD. The study aimed at clarifying the mechanisms of EA's antidepressant effects in a PSD rat model. We used middle cerebral artery occlusion to establish the rat model of PSD. Tests of sucrose preference and locomotor activity were performed to examine depressive-like behaviors. We measured malondialdehyde, GSH, SOD, IL6, IL1β, TNFα, and 5HT with ELISA. The hippocampal Shh-signaling pathway was assessed by Western blot. EA significantly decreased sucrose preference and locomotor activities of PSD rats, reduced IL6, TNFα, increased GSH, and upregulated 5HT, and also slightly reduced IL1β and malondialdehyde, all of which were measured with ELISA. The Shh-signaling pathway assessed by Western blotting was activated by EA. Those changes were inhibited by the Shh-pathway inhibitor cyclopamine. EA effectively alleviated depressive-like behaviors in PSD by suppressing inflammation and oxidative stress through activation of the Shh-signaling pathway.