Co-culture engineering: a promising strategy for production of engineered extracellular vesicle for osteoarthritis treatment

The therapeutic effects of extracellular vesicles (EVs) have been identified as a significant factor in intercellular communication in different disease treatments, including osteoarthritis (OA). Compared to the conventional approaches in treating OA, EV therapy is a non-invasive and cell-free metho...

Full description

Saved in:
Bibliographic Details
Published in:Cell communication and signaling 2024-01, Vol.22 (1), p.29-29, Article 29
Main Authors: Esmaeili, Abazar, Hosseini, Samaneh, Baghaban Eslaminejad, Mohamadreza
Format: Article
Language:English
Subjects:
Aggregates
Analysis
Antiarthritic agents
Bone marrow
Care and treatment
Cartilage
Cell Communication
Cell culture
Chondrocyte
Chondrocytes
Chondrogenesis
Co-culture engineering
Coculture Techniques
Complications and side effects
Diagnosis
Engineering
EV therapy
Extracellular matrix
Extracellular vesicle engineering
Extracellular Vesicles
Humans
Inflammation
Mesenchymal Stem Cell
Mesenchymal stem cells
Osteoarthritis
Osteoarthritis - therapy
Patient outcomes
Ratios
Review
Stem cells
Therapeutic applications
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The therapeutic effects of extracellular vesicles (EVs) have been identified as a significant factor in intercellular communication in different disease treatments, including osteoarthritis (OA). Compared to the conventional approaches in treating OA, EV therapy is a non-invasive and cell-free method. However, improving the yield of EVs and their therapeutic effects are the main challenges for clinical applications. In this regard, researchers are using the EV engineering potential to overcome these challenges. New findings suggest that the co-culture strategy as an indirect EV engineering method efficiently increases EV production and quality. The co-culture of mesenchymal stem cells (MSCs) and chondrocytes has improved their chondrogenesis, anti-inflammatory effects, and regenerative properties which are mediated by EVs. Hence, co-culture engineering by considerable systems could be useful in producing engineered EVs for different therapeutic applications. Here, we review various co-culture approaches, including diverse direct and indirect, 2D and 3D cell cultures, as well as static and dynamic systems. Meanwhile, we suggest and discuss the advantages of combined strategies to achieve engineered EVs for OA treatment.
ISSN:1478-811X
1478-811X
DOI:10.1186/s12964-023-01467-9