MicroRNA Expression Profiling by Bead Array Technology in Human Tumor Cell Lines Treated with Interferon-Alpha-2a

MicroRNAs are positive and negative regulators of eukaryotic gene expression that modulate transcript abundance by specific binding to sequence motifs located prevalently in the 3' untranslated regions of target messenger RNAs (mRNA). Interferon-alpha-2a (IFNα) induces a large set of protein co...

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Bibliographic Details
Published in:Biological procedures online 2009-07, Vol.11 (1), p.113-129, Article 113
Main Authors: Siegrist, Fredy, Singer, Thomas, Certa, Ulrich
Format: Article
Language:English
Subjects:
alpha -Interferon
Cancer cells
Cytokines
DNA microarrays
Gene expression
Gene silencing
Genetic aspects
Health aspects
Hepatoma
Interferon alpha
Interferons
Internet
Melanoma
Messenger RNA
MicroRNAs
miRNA
mRNA
Oligonucleotide Array Sequence Analysis
Physiological aspects
Reverse Transcriptase Polymerase Chain Reaction
Suppressor of Cytokine Signaling Proteins
Transcription
Tumor cell lines
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Summary:MicroRNAs are positive and negative regulators of eukaryotic gene expression that modulate transcript abundance by specific binding to sequence motifs located prevalently in the 3' untranslated regions of target messenger RNAs (mRNA). Interferon-alpha-2a (IFNα) induces a large set of protein coding genes mediating antiproliferative and antiviral responses. Here we use a global microarray-based microRNA detection platform to identify genes that are induced by IFNα in hepatoma- or melanoma-derived human tumor cell lines. Despite the enormous differences in expression levels between these models, we were able to identify microRNAs that are upregulated by IFNα in both lines suggesting the possibility that interferon-regulated microRNAs are involved in the transcriptional repression of mRNA relevant to cytokine responses.
ISSN:1480-9222
1480-9222
DOI:10.1007/s12575-009-9012-1