Cordyceps militaris Reduces Oxidative Stress and Regulates Immune T Cells to Inhibit Metastatic Melanoma Invasion

In this study, the water extract of Cordyceps militaris (Linn.) Link (CM) was used as a functional material to investigate the inhibitory mechanisms on B16F10 and lung metastatic melanoma (LMM) cells. Reducing power, chelating ability, and 2,2-diphenyl-2-picrylhydrazyl (DPPH) assays were applied for...

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Bibliographic Details
Published in:Antioxidants 2022-07, Vol.11 (8), p.1502
Main Authors: Lan, Yuan-Hong, Lu, Yun-Sheng, Wu, Ju-Yu, Lee, Hsu-Tung, Srinophakun, Penjit, Canko, Gizem Naz, Chiu, Chien-Chih, Wang, Hui-Min David
Format: Article
Language:English
Subjects:
Adenosine
Antioxidants
Apoptosis
Autophagy
B16F10
Cancer therapies
CD8 antigen
Cell growth
Cell migration
Chemotherapy
Cordyceps militaris
Cytotoxicity
Fungi
Immune system
Immunogenicity
Laboratories
lung metastatic melanoma
Lymphocytes
Lymphocytes T
Melanoma
Metastases
Metastasis
Natural products
Oxidative stress
Potassium
T cell
tumor microenvironment
Tumors
Vitamin C
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Summary:In this study, the water extract of Cordyceps militaris (Linn.) Link (CM) was used as a functional material to investigate the inhibitory mechanisms on B16F10 and lung metastatic melanoma (LMM) cells. Reducing power, chelating ability, and 2,2-diphenyl-2-picrylhydrazyl (DPPH) assays were applied for antioxidative capacities, and we obtained positive results from the proper concentrations of CM. To examine the ability of CM in melanoma proliferation inhibition and to substantiate the previous outcomes, three cellular experiments were performed via (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, MTT, a tetrazole) assay, cell migration, and invasion evaluation. The addition of CM to the incubation medium increased the number of CD8+ T cells significantly, which improved the immunogenicity. This study showed that CM exhibits various biological capabilities, including antioxidation, anti-tumor, tumor invasion suppression, and T cytotoxic cell activity promotion.
ISSN:2076-3921
2076-3921
DOI:10.3390/antiox11081502