Human papillomavirus genotypes in asymptomatic young women from public schools in Rio de Janeiro, Brazil Genótipos de papilomavírus humanos em mulheres jovens assintomáticas de escolas públicas do Rio de Janeiro, Brasil

• Published in: Revista da Sociedade Brasileira de Medicina Tropical 2010-02, Vol.43 (1), p.4-8
• Main Authors: Ledy Horto Santos Oliveira, Maria Diva Paes Lima Ferreira, Everton Faccini Augusto, Fabiana Gil Melgaço, Larissa Silva Santos, Silvia Maria Baeta Cavalcanti, Maria Luiza Garcia Rosa
• Format: Article
• Language: English
• Subjects: Cohort study; Estudo de corte; Genotypes; Genótipos; Human papillomavirus; Mulheres jovens; Papilomavírus humano; Polimorfismo do comprimento de fragmentos de restrição; Restriction fragment length polymorphism; Young women
• ISSN: 0037-8682; 1678-9849
• DOI: 10.1590/S0037-86822010000100002

INTRODUCTION: The aim of this work was to survey HPV information from a random population of young women from Rio de Janeiro, Brazil. METHODS: This cross-sectional study included cervical samples from 241 female students. To determine human papillomavirus status, polymerase chain reaction amplification was performed. HPV typing was determined by restriction fragment length polymorphism analysis. Demographic data, life style, sexual and gynecological history were obtained through use of a structured questionnaire. RESULTS: The average age of the women was 19.6 years-old (SD=3.4 years). HPV prevalence was 27.4%. Nineteen different HPV genotypes were detected, including 13 high risk types. HPV 16 was the most prevalent type (6.2%), followed by 31 (4.1 %) and 66 (3.7%). Most of the oncogenic types belonged to the A9 species (28/48). The frequency of women infected by at least one oncogenic type was significantly higher than those only infected by low risk types (18.7% versus 7.5%). Cervical changes were detected in 12.5% of the sample and were significantly linked to infection with HPV types of the A9 species. Demographic variables, sexual initiation, or number of sexual partners were not associated with HPV prevalence, variety of HPV genotypes or oncogenic types. CONCLUSIONS: The relative frequency of HPV genotypes other than vaccine types in young females should be taken into account when evaluating vaccination strategies. Due to the high prevalence of HPV infection among the population studied, implementation of sex education in schools, promotion of condom use and an organized screening program to prevent cervical cancer must be encouraged for this age group.INTRODUÇÃO: O objetivo deste trabalho foi investigar a infecção por HPV em uma população randômica de mulheres jovens no estado do Rio de Janeiro, Brasil. MÉTODOS: Este estudo incluiu amostras cervicais de 241 jovens. A detecção de papilomavírus humanos foi realizada pela reação da cadeia da polimerase e a tipificação deste vírus determinada pelo polimorfismo do comprimento do fragmento de restrição. Dados demográficos, estilo de vida, história sexual e ginecológica foram obtidos através de um questionário. A média de idade das mulheres foi 19,6 anos (SD=3,4). RESULTADOS: A prevalência de HPV foi de 27,4% e 19 diferentes tipos foram detectados, incluindo 13 oncogênicos. O HPV 16 foi o tipo mais prevalente (6,2%), seguido do 31 (4,1%) e do 66 (3,7%). A maioria dos tipos oncogênicos pertencia à espécie A