PICROSIRIUS RED AND MASSON’S TRICHROME STAINING TECHNIQUES AS TOOLS FOR DETECTION OF COLLAGEN FIBERS IN THE SKIN OF DOGS WITH ENDOCRINE DERMATOPATHOLOGIES

• Published in: Ciência animal brasileira 2019, Vol.20, p.1-10
• Main Authors: Costa, Glícia Meneses, Araujo, Steffi Lima, Xavier Júnior, Francisco Antônio Félix, Morais, Glayciane Bezerra de, Silveira, João Alison de Moraes, Viana, Daniel de Araújo, Evangelista, Janaina Serra Azul Monteiro
• Format: Article
• Language: English
• Subjects: VETERINARY SCIENCES
• ISSN: 1518-2797; 1809-6891; 1809-6891
• DOI: 10.1590/1089-6891v20e-55398

Abstract Canine endocrinopathies, such as hypothyroidism and hyperadrenocortism,induce typical dermatological alterations. Collagen fibers are significant for the maintenance of structural integrity,as well as in the determination of tissue function. This study aimed at assessing the coloration caused by Picrosirius Red staining under circular polarization and Masson Trichrome staining, as tools to quantify the total collagen in the skin of dogs exhibiting endocrine dermatopathies. Skin samples taken from dogs with hypothyroidism and hyperadrenocorticism were stained using Hematoxylin and Eosin (HE), Masson’s Trichrome (MT) and Picrosirius Red (PSR). The animals with hyperadrenocorticism revealed a higher percentage of collagen area than did the animals with hypothyroidism PSRp (hypothyroidism = 43.76 ± 0.8255 and hyperadrenocorticism = 47.08 ± 0.8584). The percentage of the collagen area using MT between the groups is given in parenthesis (hypothyroidism = 52.06 ± 0.9371, hyperadrenocorticism = 61 ± 0.7529 and control = 56.88 ± 0.64) (p