Transcriptome of Nosema ceranae and Upregulated Microsporidia Genes during Its Infection of Western Honey Bee ( Apis mellifera )

• Published in: Insects (Basel, Switzerland) Switzerland), 2022-08, Vol.13 (8), p.716
• Main Authors: Li, Yi-Hsuan, Chang, Zih-Ting, Yen, Ming-Ren, Huang, Yu-Feng, Chen, Tzu-Han, Chang, Ju-Chun, Wu, Ming-Cheng, Yang, Yu-Liang, Chen, Yue-Wen, Nai, Yu-Shin
• Format: Article
• Language: English
• Subjects: Apiculture; Apis mellifera; Bees; common DEGs; Cytoplasm; Cytosol; Data processing; Environmental information; Gene expression; Genes; Genomes; Honey; Infections; Information processing; Metabolism; Microbiota; Microsporidia; Nosema ceranae; Parasites; Pesticides; Physiology; Protein biosynthesis; Protein synthesis; Proteins; Splicing; Spores; stage-specific genes; Structural proteins; Transcription factors; transcriptome; Transcriptomes; Transcriptomics
• ISSN: 2075-4450; 2075-4450
• DOI: 10.3390/insects13080716

is one of the fungal parasites of . It causes physical and behavioral effects in honey bees. However, only a few studies have reported on gene expression profiling during infection. In this study, the transcriptome profile of mature spores at each time point of infection (5, 10, and 20 days post-infection, d.p.i.) were investigated. Based on the transcriptome and expression profile analysis, a total of 878, 952, and 981 differentially expressed genes (DEGs) (fold change ≥ 2 or ≤ -2) were identified in spores (NcSp) at 5 d.p.i., 10 d.p.i., and 20 d.p.i., respectively. Moreover, 70 upregulated genes and 340 downregulated genes among common DEGs (so-called common DEGs) and 166 stage-specific genes at each stage of infection were identified. The Gene Ontology (GO) analysis indicated that the DEGs and corresponding common DEGs are involved in the functions of cytosol (GO:0005829), cytoplasm (GO:0005737), and ATP binding (GO:0005524). Furthermore, the pathway analysis found that the DEGs and common DEGs are involved in metabolism, environmental information processing, and organismal systems. Four upregulated common DEGs with higher fold-change values, highly associated with spore proteins and transcription factors, were selected for validation. In addition, the stage-specific genes are highly involved in the mechanism of pre-mRNA splicing according to GO enrichment analysis; thus, three of them showed high expression at each d.p.i. and were also subjected to validation. The relative gene expression levels showed a similar tendency as the transcriptome predictions at different d.p.i., revealing that the gene expression of during infection may be related to the mechanism of gene transcription, protein synthesis, and structural proteins. Our data suggest that the gene expression profiling of at the transcriptomic level could be a reference for the monitoring of nosemosis at the genetic level.