Evaluation of the Fecal Proteome in Healthy and Diseased Cheetahs ( Acinonyx jubatus ) Suffering from Gastrointestinal Disorders

Fecal proteomics allows for the identification of proteins and peptides present in stools and is useful in finding possible new biomarkers for diagnosing and/or monitoring gastrointestinal (GI) disorders. In the present study, we investigated the fecal proteome in healthy and diseased cheetahs ( )....

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Bibliographic Details
Published in:Animals (Basel) 2022-09, Vol.12 (18), p.2392
Main Authors: Mangiaterra, Sara, Vincenzetti, Silvia, Rossi, Giacomo, Marchegiani, Andrea, Gavazza, Alessandra, Petit, Thierry, Sagratini, Gianni, Ricciutelli, Massimo, Cerquetella, Matteo
Format: Article
Language:English
Subjects:
Acids
Acinonyx jubatus
Albumins
Animals
cheetah
Collagen
Connectin
Diagnostic systems
Diarrhea
Electrophoresis
Endoscopy
Fc receptors
fecal proteomics
Feces
Gastrointestinal diseases
gastrointestinal disorder
Helicobacter
Helicobacter spp
Immunoglobulin G
Invasiveness
Isoforms
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Monitoring
Peptides
Potassium
Protein disulfide-isomerase
Proteins
Proteomes
Proteomics
Software
Telemedicine
Transthyretin
Vomiting
Weight loss
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Summary:Fecal proteomics allows for the identification of proteins and peptides present in stools and is useful in finding possible new biomarkers for diagnosing and/or monitoring gastrointestinal (GI) disorders. In the present study, we investigated the fecal proteome in healthy and diseased cheetahs ( ). Captive individuals of this species frequently show gastrointestinal disorders characterized by recurrent episodes of diarrhea, rare episodes of vomiting and weight loss, associated with spp. infection. Fecal proteomic evaluation has been performed by two-dimensional electrophoresis followed by liquid chromatography-tandem mass spectrometry. In healthy cheetahs, the results showed the presence of the following proteins: collagen alpha-1 (II) chain, transthyretin, IgG Fc-binding protein, titin, dystonin, isopentenyl-diphosphate Delta-isomerase 1, sodium/potassium-transporting ATPase subunit alpha-1 and protein disulfide-isomerase A6. The presence of albumin isoforms was found only in diseased cheetahs. The present paper reports the study of the fecal proteome in the cheetah, evidences some differences between healthy and diseased patients and confirms, once again, the potential of fecal proteomics for the study of the GI environment, with promising developments regarding the identification of new diagnostic/monitoring markers.
ISSN:2076-2615
2076-2615
DOI:10.3390/ani12182392