Molecular Detection of Leishmania spp. in Skin and Blood of Stray Dogs from Endemic Areas of Cutaneous Leishmaniasis in Saudi Arabia

• Published in: Iranian journal of parasitology 2019-04, Vol.14 (2), p.231-239
• Main Authors: Alanazi, Abdullah D, Puschendorf, Robert, Alyousif, Mohamed S, Al-Khalifa, Mohamed S, Alharbi, Samir A, Al-Shehri, Zafer S, Said, Ashraf E, Alanazi, Ibrahim O, Al-Mohammed, Hamdan I, Alraey, Yasser A
• Format: Article
• Language: English
• Subjects: Dogs; kDNA; Leishmania; Original; Parasitic diseases; Protozoa; Saudi Arabia
• ISSN: 1735-7020; 2008-238X
• DOI: 10.18502/ijpa.v14i2.1135

Dogs can act as reservoirs of canine leishmaniasis, caused by species. The aims of this study were to determine the prevalence of canine leishmaniasis using a PCR technique among stray dogs living in three provinces of Saudi Arabia, Riyadh, Al-Ahsa Oasis and Al-Qaseem, where the disease is endemic; and to identify and document different to species levels. This cross-sectional investigation was conducted, from Mar 2016 to Apr 2018, in three parts of Saudi Arabia: Central province (Riyadh), Eastern province (Al-Ahsa Oasis) and Al-Qaseem province. Blood samples were collected from 526 dogs; 40 presented cutaneous nodules so were suspected clinically of cutaneous leishmaniasis. Biopsy tissue collections and parasite cultures were performed. A generic kDNA was performed using different primers for differentiation. All blood samples were negative for infection by molecular analysis, though forty dogs had thick cutaneous lesions in different parts of their body. Four dogs' skin lesions were associated with dermatitis, splenomegaly and lymphadenomegaly. Parasite culture was used to diagnose cutaneous leishmaniasis, identifying 31/40 (77.5%) positive samples. Overall, of 526 samples, the prevalence of and was found to be 4% and 1.9%, respectively. Gender and age had a significant effect on prevalence: ( =0.0212 and 0.0357), respectively. This was the first molecular study of dog leishmaniasis from Saudi Arabia of dogs confirmed to have cutaneous leishmaniasis. Further epidemiological and molecular investigations of domestic and wild canine infections with , and in endemic and nonendemic areas of Saudi Arabia are required, for leishmaniasis control.