Interleukin-12 as a biomarker of the beneficial effects of food restriction in mice receiving high fat diet or high carbohydrate diet

• Published in: Brazilian journal of medical and biological research 2018-01, Vol.51 (12), p.e7900-e7900
• Main Authors: de Almeida-Souza, C B, Antunes, M M, Godoy, G, Schamber, C R, Silva, M A R C P, Bazotte, R B
• Format: Article
• Language: English
• Subjects: Animal Nutritional Physiological Phenomena - physiology; Animals; Anti-inflammatory cytokines; Appetite (Psychophysiology); BIOLOGY; Biomarkers - blood; Body Weight; Caloric restriction; Caloric Restriction - methods; Control; Cytokines - blood; Diet, Carbohydrate Loading - methods; Diet, High-Fat - methods; Food Deprivation - physiology; Health aspects; High fat diet; Immunoassay - methods; Interleukin-12; Interleukin-12 - blood; MEDICINE, RESEARCH & EXPERIMENTAL; Mice; Nutrition; Pro-inflammatory cytokines; Reference Values; Serum cytokines; Time Factors
• ISSN: 0100-879X; 1414-431X; 1414-431X
• DOI: 10.1590/1414-431X20187900

The impact of food restriction (FR) during 56 days on serum levels of cytokines in mice fed a high-fat diet (HFD) or high-carbohydrate diet (HCD) were evaluated. The amount of food was reduced 50% for HFD-FR and HCD-FR groups compared to mice receiving free access to HFD (HFD group) or HCD (HCD group). We quantified the serum levels of basic fibroblast growth factor, granulocyte-macrophage colony-stimulating factor, inducible protein 10, interferon γ, interleukin 1α (IL-1α), IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12, IL-13, IL-17, keratinocyte chemoattractant, macrophage inflammatory protein-1α, monocyte chemotactic protein 1, monokine induced by IFN-γ, and tumor necrosis factor α. Only IL-12 levels were lower (P