The Cactus ( Opuntia ficus-indica ) Cladodes and Callus Extracts: A Study Combined with LC-MS Metabolic Profiling, In-Silico, and In-Vitro Analyses

(OF) phytochemicals have received considerable attention because of their health benefits. However, the structure-activity relationship between saponin and flavonoid antioxidant compounds among secondary metabolites has rarely been reported. In a molecular docking study, selected compounds from both...

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Bibliographic Details
Published in:Antioxidants 2023-06, Vol.12 (7), p.1329
Main Authors: Nam, Dong-Geon, Yang, Hee-Sun, Bae, Ui-Jin, Park, Eunmi, Choi, Ae-Jin, Choe, Jeong-Sook
Format: Article
Language:English
Subjects:
Acids
Anti-inflammatory agents
Antioxidants
Cactus
Callus
Cell culture
ethanolic extract
Flavonoids
Food
Global warming
Human beings
Inflammation
Influence on nature
Ions
Kinases
LC-MS
Ligands
MAP kinase
Metabolism
molecular docking
Opuntia ficus-indica
Opuntia ficus-indica cladodes
phytochemical
Phytochemicals
Prostaglandin E2
Proteins
Rutin
Saponins
Secondary metabolites
Software
TLR4 protein
Toll-like receptors
Tumor necrosis factor-α
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Summary:(OF) phytochemicals have received considerable attention because of their health benefits. However, the structure-activity relationship between saponin and flavonoid antioxidant compounds among secondary metabolites has rarely been reported. In a molecular docking study, selected compounds from both callus (OFC) and OF ethanol extract were found to be involved in Toll-like receptor 4 and mitogen-activated protein kinase (MAPK) signaling pathways. High affinity was specific for MAPK, and it was proposed to inhibit the oxidative and inflammatory responses with poricoic acid H (-8.3 Kcal/mol) and rutin (-9.0 Kcal/mol). The pro-inflammatory cytokine factors at a concentration of 200 μg/mL were LPS-stimulated TNF-α (OFC 72.33 ng/mL, OF 66.78 ng/mL) and IL-1β (OFC 49.10 pg/mL, OF 34.45 pg/mL), both of which significantly decreased OF ( < 0.01, < 0.001). Taken together, increased NO, PGE , and pro-inflammatory cytokines were significantly decreased in a dose-dependent manner in cells pretreated with OFC and the OF extract ( < 0.05). These findings suggest that OFC and OF have important potential as natural antioxidant, anti-inflammatory agents in health-promoting foods and medicine.
ISSN:2076-3921
2076-3921
DOI:10.3390/antiox12071329