High-throughput microarray reveals the epitranscriptome-wide landscape of m6A-modified circRNA in oral squamous cell carcinoma

Emerging transcriptome-wide high-throughput screenings reveal the landscape and functions of RNAs, such as circular RNAs (circRNAs), in human cancer. In addition, the post-transcriptional RNA internal modifications, especially N.sup.6-methyladenosine (m.sup.6A), greatly enrich the variety of RNAs me...

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Published in:BMC genomics 2022-08, Vol.23 (1), p.1-611, Article 611
Main Authors: Zhao, Wei, Liu, Jingwen, Wu, Jie, Ma, Xiaozhou, Wang, Xi, Zhang, Leyu, Han, Zhe, Yang, Jianming, Cui, Yameng, Hu, Xin, Deng, Jiayin
Format: Article
Language:English
Subjects:
3' Untranslated regions
Cancer
Circular RNA
Diagnosis
Enzymes
Exons
Gene sequencing
Genes
Genetic aspects
Genetic transcription
Genomics
Health aspects
Immunoprecipitation
m6A-circRNAs
Medical prognosis
MeRIP-Seq
N6-Methyladenosine
Oral cancer
Oral carcinoma
Oral squamous cell carcinoma
Post-transcription
RNA
RNA modification
Squamous cell carcinoma
Stop codon
Transcriptomes
Tumorigenesis
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Summary:Emerging transcriptome-wide high-throughput screenings reveal the landscape and functions of RNAs, such as circular RNAs (circRNAs), in human cancer. In addition, the post-transcriptional RNA internal modifications, especially N.sup.6-methyladenosine (m.sup.6A), greatly enrich the variety of RNAs metabolism. However, the m.sup.6A modification on circRNAs has yet to be addressed. Here, we report an epitranscriptome-wide mapping of m.sup.6A-modified circRNAs (m.sup.6A-circRNA) in oral squamous cell carcinoma (OSCC). Utilizing the data of m.sup.6A methylated RNA immunoprecipitation sequencing (MeRIP-seq) and m.sup.6A-circRNAs microarray, we found that m.sup.6A-circRNAs exhibited particular modification styles in OSCC, which was independent of m.sup.6A-mRNA. Besides, m.sup.6A modification on circRNAs frequently occurred on the long exons in the front part of the coding sequence (CDS), which was distinct from m.sup.6A-mRNA that in 3'-UTR or stop codon. In conclusion, our work preliminarily demonstrates the traits of m.sup.6A-circRNAs, which may bring enlighten for the roles of m.sup.6A-circRNAs in OSCC.
ISSN:1471-2164
1471-2164
DOI:10.1186/s12864-022-08806-z