Knockout of PERK protects rat Müller glial cells against OGD-induced endoplasmic reticulum stress-related apoptosis

The pathological basis for many retinal diseases, retinal ischemia is also one of the most common causes of visual impairment. Numerous ocular diseases have been linked to Endoplasmic reticulum(ER)stress. However, there is still no clear understanding of the relationship between ER stress and Müller...

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Bibliographic Details
Published in:BMC ophthalmology 2023-06, Vol.23 (1), p.286-286, Article 286
Main Authors: Wang, Xiaorui, Zhu, Xinxing, Huang, Guangqian, Wu, Lili, Meng, Zhiyong, Wu, Yuyu
Format: Article
Language:English
Subjects:
Alzheimer's disease
Animals
Apoptosis
Autophagy
Caspase-12
Cells
Cholecystokinin
Endoplasmic reticulum
Endoplasmic Reticulum Stress
Ependymoglial Cells
Eye diseases
Flow cytometry
Glial cells
Glucose
Homeostasis
Hypoxia
Immunofluorescence
Initiation factor eIF-2
Ischemia
Metabolism
Mueller cells
Müller glia
Ophthalmology
Oxygen
Oxygen-glucose deprivation
Pathogenesis
Protein synthesis
Proteins
Rats
Rats, Sprague-Dawley
Reagents
Retina
Signal Transduction
Tau protein
Western blotting
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Summary:The pathological basis for many retinal diseases, retinal ischemia is also one of the most common causes of visual impairment. Numerous ocular diseases have been linked to Endoplasmic reticulum(ER)stress. However, there is still no clear understanding of the relationship between ER stress and Müller glial cells during retinal ischemia and hypoxia. This study examined the effects of ER stress on autophagy and apoptosis-related proteins, as well as the microtubule-related protein tau in rMC-1 cells. rMC-1 cells were cultured in vitro. RT-PCR、immunofluorescence and Western blotting revealed the expression levels of associated mRNAs and proteins, and the CCK-8 and flow cytometry assays detected cell apoptosis. The results showed that under OGD(Oxygen-glucose deprivation) conditions, the number of rMC-1 cells was decreased, the PERK/eIF2a pathway was activated, and the expressions of p-tau, LC3、Beclin1 and Caspase-12 proteins were increased. After the PERK knockout, the expression of the above proteins was decreased, and the apoptosis was also decreased. According to the findings of this study, specific downregulation of PERK expression had an anti-apoptotic effect on OGD-conditioned rMC-1 cells. There is a possibility that this is one of the mechanisms of MG cell apoptosis during retinal ischemic injury.
ISSN:1471-2415
1471-2415
DOI:10.1186/s12886-023-03022-z