Resveratrol ameliorates 2,4-dinitrofluorobenzene-induced atopic dermatitis-like lesions through effects on the epithelium

• Published in: PeerJ (San Francisco, CA) CA), 2016-03, Vol.4, p.e1889-e1889, Article e1889
• Main Authors: Caglayan Sozmen, Sule, Karaman, Meral, Cilaker Micili, Serap, Isik, Sakine, Arikan Ayyildiz, Zeynep, Bagriyanik, Alper, Uzuner, Nevin, Karaman, Ozkan
• Format: Article
• Language: English
• Subjects: Allergy and Clinical Immunology; Analysis; Animal experimentation; Apoptosis; Atopic dermatitis; Dermatology; Epithelium; Immunology; Interleukins; Mice; Resveratrol; Skin; Treatment
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.1889

Background. Resveratrol is a natural polyphenol that exhibits anti-inflammatory effects. The aim of this study was to investigate the effects of resveratrol treatment on epithelium-derived cytokines and epithelial apoptosis in a murine model of atopic dermatitis-like lesions. Material and Methods. Atopic dermatitis-like lesions were induced in BALB/c mice by repeated application of 2,4-dinitrofluorobenzene to shaved dorsal skin. Twenty-one BALB/c mice were divided into three groups: group I (control), group II (vehicle control), and group III (resveratrol). Systemic resveratrol (30 mg/kg/day) was administered repeatedly during the 6th week of the experiment. After the mice had been sacrificed, skin tissues were examined histologically for epithelial thickness. Epithelial apoptosis (caspase-3) and epithelium-derived cytokines [interleukin (IL)-25, IL-33, and thymic stromal lymphopoietin (TSLP)] were evaluated immunohistochemically. Results. Epithelial thickness and the numbers of IL-25, IL-33, TSLP and caspase-3-positive cells were significantly higher in group II compared to group I mice. There was significant improvement in epithelial thickness in group III compared with group II mice (p < 0.05). The numbers of IL-25, IL-33, and TSLP-positive cells in the epithelium were lower in group III than in group II mice (p < 0.05). The number of caspase-3-positive cells, as an indicator of apoptosis, in the epithelium was significantly lower in group III than in group II mice (p < 0.05). Conclusion. Treatment with resveratrol was effective at ameliorating histological changes and inflammation by acting on epithelium-derived cytokines and epithelial apoptosis.