Molecular characterization of rabies virus isolated from non-haematophagous bats in Brazil

Rabies is an important zoonosis that causes thousands of deaths worldwide each year. Although the terrestrial cycle, mainly transmitted by dogs, is controlled in Brazil, the aerial cycle remains a serious public health issue, besides the economic problem. In the aerial cycle, the haematophagous bat...

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Published in:Revista da Sociedade Brasileira de Medicina Tropical 2011-11, Vol.44 (6), p.678-683
Main Authors: Albas, Avelino, Campos, Angélica Cristine de Almeida, Araujo, Danielle Bastos, Rodrigues, Camila Seabra, Sodré, Miriam Martos, Durigon, Edison Luiz, Favoretto, Silvana Regina
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - blood
Antibodies, Viral - blood
Brazil
Caracterização antigênica
Caracterização genética
Cattle
Central nervous system
Chiroptera - classification
Chiroptera - virology
Genetic variance
Morcegos
Phylogeny
Public health
Rabies virus - genetics
Rabies virus - immunology
Rabies virus - isolation & purification
Raiva
Reverse Transcriptase Polymerase Chain Reaction
TROPICAL MEDICINE
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Summary:Rabies is an important zoonosis that causes thousands of deaths worldwide each year. Although the terrestrial cycle, mainly transmitted by dogs, is controlled in Brazil, the aerial cycle remains a serious public health issue, besides the economic problem. In the aerial cycle, the haematophagous bat Desmodus rotundus is the main source of infection, where several different species of non-haematophagous bats can be infected and can transmit the virus. The aim of this work was to study the epidemiological pattern of rabies using antigenic characterization with monoclonal antibodies and genetic characterization by reverse-transcriptase polymerase chain reaction followed by sequencing and phylogenetic analysis of non-haematophagous bats' and herbivorous animals' central nervous system samples from the western region of the State of São Paulo, Brazil. From 27 samples, 3 antigenic variants were identified: AgV-3, AgV-4, and AgV-6; and from 29 samples, 5 different clusters were identified, all belonging to the rabies virus species. Although only non-haematophagous bats were evaluated in the studied region, the majority of samples were from antigenic and genetic variants related to haematophagous bats Desmodus rotundus. Samples from the same antigenic variant were segregated in more than one genetic cluster. This study demonstrated the diversity of rabies virus genetic lineages presented and circulating in non-haematophagous bats in the studied region.
ISSN:0037-8682
1678-9849
1678-9849
0037-8682
DOI:10.1590/S0037-86822011000600006