Concentrated ambient fine particles exposure affects ovarian follicle development in mice

Ambient fine particles (PM2.5) are known to cause various reproductive and developmental diseases. However, the potential mechanisms of PM2.5 exposure induced female reproductive damage remain unclear. Four weeks old female C57BL/6 J mice were exposed to filtered air (FA, n = 10) or concentrated amb...

Full description

Saved in:
Bibliographic Details
Published in:Ecotoxicology and environmental safety 2022-02, Vol.231, p.113178-113178, Article 113178
Main Authors: Yang, Mingjun, Tian, Fang, Tao, Shimin, Xia, Minjie, Wang, Yuzhu, Hu, Jingying, Pan, Bin, Li, Zhouzhou, Peng, Renzhen, Kan, Haidong, Xu, Yanyi, Li, Weihua
Format: Article
Language:English
Subjects:
Aerosols
Air Pollutants - analysis
Air Pollutants - toxicity
Ambient PM2.5
Animals
Apoptosis
Female
Follicular Atresia
Inflammatory response
Mice
Mice, Inbred C57BL
Ovarian dysfunction
Ovarian Follicle
Particulate Matter - toxicity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Ambient fine particles (PM2.5) are known to cause various reproductive and developmental diseases. However, the potential mechanisms of PM2.5 exposure induced female reproductive damage remain unclear. Four weeks old female C57BL/6 J mice were exposed to filtered air (FA, n = 10) or concentrated ambient PM2.5 (CAP, n = 10) using a versatile aerosol concentration enrichment system. After 9 weeks of the exposure, mice were sacrificed under sevoflurane anesthesia and tissue samples were collected. Immunohistochemical analysis, enzyme-linked immunosorbent assay, quantitative polymerase chain reaction, and RNA-sequencing were performed to analyze the effects of PM2.5 exposure on follicle development and elucidate its potential mechanisms. Chronic PM2.5 exposure resulted in follicular dysplasia. Compared to the FA-exposed group, follicular atresia in the CAP-exposed mice were significantly increased. Further studies confirmed that CAP induced apoptosis in granulosa cells, accompanied by a distortion of hormone homeostasis. In addition, RNA-sequencing data demonstrated that CAP exposure induced the alteration of ovarian gene expressions and was associated with inflammatory response. Chronic exposure to CAP can induce follicular atresia, which was associated with hormone modulation and inflammation. [Display omitted] •PM2.5 exposure induced follicular atresia in female mice.•PM2.5 exposure promoted granulosa cell apoptosis in the atretic follicles.•PM2.5 exposure disturbed hormone homeostasis.•PM2.5 exposure triggered inflammatory response in mouse ovary.
ISSN:0147-6513
1090-2414
DOI:10.1016/j.ecoenv.2022.113178